Hi, I have a problem with my mouse Adipose MSC primary culture?

I am working on mouse adipose mesanchymal stem cells. After removing the mouse peritoneal fat and extracting, I do the primary culture, I used DMEM F12 medium with 15% FBS, but with this, the...

13 February 2021 9,838 1 View

How to estimate total electron content using GPS data (with MATLAB)?

06 January 2021 1,110 2 View

How can I find the area of contour with magnitude less than specific number?

Hello everyone I have results from fluent and I want to determine area with magnitude less than specific number for example for velocity or TKE. and also how can I determine Max and min diameter...

02 November 2020 5,863 3 View

Why residuals stay at the same range in vof model in Fluent and it doesn't converge?

hi I'm modeling a 3d channel with vof , k-epsilon RNG with non-equilibrium wall functions , for bc I have velocity inlet, pressure outlet and for top of the domain I have used pressure outlet...

21 August 2020 5,926 7 View

How I can obtain the cartesian coordinates of a complex without single crystals for gaussian calculations?

When the single crystals of complex are not formed, how we can obtain cartesian coordinates for Gaussian calculations? We want to predict kind of interaction or coordination of manganese to amino...

18 August 2020 8,146 3 View

I am confused about the editor's request (I had submitted a manuscript and then major revision was requested). Can anybody help me?

I had submitted a manuscript to a journal. After a while, they requested major revisions. The end of their request, they mentioned that: "You will be unable to make your revisions on the...

09 August 2020 5,436 7 View

Which algorithm (Sobel, Perwitt, Roberts, Canny and LOG algorithm) is more capable of finding noisy image edges?

The edge detection algorithms are designed to respond to sharp edges which can be caused by noisy pixels. In image analysis edge detection plays an important role and it is one of the traditional...

05 August 2020 2,543 1 View

What is the best way of Evaluation Performance for Multiclass Data?

For classification problems, classifier performance is typically defined according to the confusion matrix associated with the classifier. Based on the entries of the matrix, it is possible to...

19 May 2020 3,472 4 View

Best EEG dataset for sleep stage classification?

I am looking for a best dataset with signals of EEG that is used for sleep stage classification.

12 May 2020 5,935 3 View

What are the public biosignals (ECG, etc) datasets of COVID-19 patients?

I am researching QOVI-19 and need a database that contains biosignal data such as electrocardiograms. Is there any dataset?

12 May 2020 9,274 3 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

Recalcitrant seed short-mid storage method?

Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...

25 February 2021 2,442 1 View

How can plant phytochemistry be included in discussions about whole plant traits?

I am using the concept of plant functional traits (Cornellisen et al., 2003) in a paper. Considering descriptions for whole plant traits (life history; life form; growth form; plant height;...

24 February 2021 6,831 5 View

Is there a suitable way to confine cell attachment area inside a multi-well plate?

Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...

22 February 2021 7,127 4 View

What is the best way to isolate Hydrolysable tannins on Sephadex column?

I am currently preparing to isolate polyphenolics (Hydrolysable tannins) from aqueous extract of a plant. I want to set up a small sephadex column to guide the major isolation on a laerge...

18 February 2021 1,455 3 View

Is it possible to use maize mature seed to do transformation for maize transgenic study?

Almost all research reports which i ever seen work on Early mature embryo for Agrobacterium mediated maize transformation. In other grass families like rice, we can use mature seed and what do...

17 February 2021 1,434 13 View

Does calculating the interaction index between a drug and a food matrix make sense?

Dear all, I'm conducting the revisions of some of my manuscript, with is the impact of the combination of Aspirin and a fruit juice in colorectal cancer cells. Two of the reviewers have asked to...

16 February 2021 3,141 3 View

Dealing with BMDM seeding and RNA extraction ?

biomedical master student here , i am dealing with mice BMDM and would like some help , i seed 0.5 x 10^6 cells/per well in a 6 well plate but when i extract the RNA i get a very low yeild , i...

15 February 2021 1,102 5 View

Is secondary metabolite production more at vegetative growth stage or during reproductive stage?

Hi, I am working on the effect of heavy metals on a medicinal plant by pot experimentation. Analyzing the effect of heavy metals on secondary metabolites is one of the parameters. I will be...

11 February 2021 6,683 6 View

Why do my polyphenol measurements fail?

In order to measure the polyphenols in broccoli, we prepared a reagent for measurement using the following procedure. 1. Extract the crushed plant with 70% methanol. 2. Mix 1 ml of the extract...

07 February 2021 845 4 View