What is the exact function of Ca++ and Mg++ in the preparation of competent cells used for transformation?

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How to avoid mucilage from petals of Alcea rosa or Hibiscus rosa?

While crushing the petals of Althea rosa in required extraction buffer lot of mucilage comes out in supernatant which does not allow the enzyme to disperse and act on substrate. How to resolve...

06 July 2018 2,836 0 View

Can anyone provide few milligrams of the chemical, "ALOESONE"?

Unfortunately, the chemical is not available with any of the companies I have checked out. Moreover, the work related to it is prime part of my PhD objectives. I have mailed to immense number of...

03 April 2014 4,251 0 View

What is the best method for checking the integrity of newly synthesized cDNA?

Literally, checking some primers of constitutive genes may work well. However, I still feel that there is some ambiguity.

03 April 2013 3,872 11 View

How to back transform the results generated from analyses using log transformed with In(X+1) data?

I am conducting my analysis using SPSS. I log transformed my data using In(X+1) as my data contain zero values. However, when I want to back transform the regression coefficients generated from my...

31 July 2024 7,860 3 View

What exactly is it about biotechnology and medical research?

I'm very interested in biotechnology and medical research and I'd like to meet a professional here and talk about it together

28 July 2024 3,798 4 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Swerling Characteristic functions?

Hello!!! I want to implement the Swerling characteristics functions (CF) directly in MATLAB without using its Fourier integral pairs...the Swerling CFs are actually Laplace Transform of the signal...

23 July 2024 4,925 1 View

Radar Detection Probabilities?

Currently I need to calculate detection probabilities (PD) from RCS data. Beta distribution parameters for this RCS data are calculated and will be used in Swerling0 Equation. The idea is based on...

22 July 2024 2,851 0 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

Do you know CUSABIO Biotechnology Scholarships for Students 2024?

I am pleased to announce the CUSABIO Biotechnology Scholarships for Students 2024! This scholarship aims to support outstanding students pursuing studies in the field of biotechnology. Here are...

22 July 2024 5,790 1 View

Radar Detection Probabilities using beta distributed Scattering Cross section?

Currently I need to calculate detection probabilities (PD) from radar cross section (RCS) data. Beta distribution parameters for this RCS data are calculated and will be used in Swerling0...

22 July 2024 868 0 View

Bimodal Distribution, Split or Transform for ANOVA?

Hi, I have some experiments where different nutrients/fertilisers (including but not limited to N) were applied to plants. Unsurprisingly the data produced is bimodal. The populations are...

18 July 2024 7,305 1 View

How to distinguish the DRX prior austenite grains (PAG) by EBSD of the martensite transformed from PAG （Ausforming）?

During the heavy warm rolling process, austenite undergoes dynamic recrystallization (either DDRX or CDRX), and then transforms into martensite upon quenching. Is there a way to distinguish from...

11 July 2024 7,602 1 View