What is the effect of a cluttered environment on mental health and well being?

21 March 2023 0 2K Report

What is the effect of a cluttered environment on the well being of children growing up?

Living with the things others threw out, how does that affect the mental well being of children?

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How effective is online therapy in the social work environment?

Is it possible to effectively address social needs through online therapy? What types of online therapy does social workers offer online?

20 March 2023 5,712 0 View

Why do my KRasG12D mice injected with Tamoxifen not develop PDAC?

We have been working on PDAC for 2-3 years now but since a couple of months, my mice do not develop PDAC anymore. This is the protocol I follow: - 2 month old mice ElaCreER / LSL-KrasG12D from...

23 May 2018 1,099 1 View

What is the best positive control for ROS measurement with CellRox ?

Because cells I'm using express GFP, I use CellRox for ROS detection. Unfortunately, I'm not able to see differences between my conditions as expected. I need to know if CellRox could detect ROS...

23 May 2016 4,209 2 View

Is it possible to use too much lysis buffer?

We are using a mechanical pipetting machine to pipette lysed cells in buffer into a 96 well plate from a 24. The protocol asks for 100 uL of lysis buffer to be used in the 24 well plate, but this...

15 May 2016 7,077 6 View

Is there a benefit to using different pH indicators for carbon utilization test?

Hello all, I am interested in performing a carbon utilization test for several L. monocytogenes strains. I have encountered various protocols and I'm unsure which would work best. Some use phenol...

14 January 2016 9,467 1 View

How can I obtain a homozygous gene KO using CRISPR-Cas9 in iPS cells?

I want to generate a KO of my favourite gene using CRISPR-Cas9 in iPS cells. I already obtained an heterozygous clone but I was wondering if I could transfect this clone again to improve chances...

15 October 2015 1,070 3 View

Sonication vs homogenization alone?

Our lab is looking into Western Blot alternatives to the classic membrane method, and are developing a protocol for mouse brain tissue preparation. We have whole tissue that we are determining the...

01 September 2015 1,213 5 View

Does matter flow through ecosystems and energy is recycled and how does the flow of matter and energy compare in the living world?

03 August 2024 607 2 View

Interested in a SCOPUS collaboration?

Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope of...

02 August 2024 8,572 0 View

Broca’s area must be intact for the learning of new movement sequences?

When the eyes of a person are damaged this causes complete blindness. Likewise, when Wernicke’s and Broca’s areas of neocortex are damaged this causes complete aphasia, losing the ability to...

01 August 2024 6,744 2 View

How do living organisms play a role in the water cycle and why is nonpoint source pollution potentially more harmful than point source pollution?

01 August 2024 7,061 2 View

How Do Project Data Analytics and AI Advance Quality 4.0 in Construction Project Management?

As the construction industry advances, the integration of Project Data Analytics and Artificial Intelligence (AI) is becoming increasingly crucial in project management. These technologies are...

31 July 2024 6,484 1 View

Would you be willing to take a look at interview questions for a research study?

25 July 2024 6,938 3 View

How apply Kao Residual Cointegration Test in eviews?

Kao's panel cointegration tests , is there anyone willing to explain me the eviews-9 output for the Kao's panel cointegration tests?

23 July 2024 5,051 4 View

What it is, that we do not know about the possible Social Impact of the AI on Society. Education, Human Computer Interaction and the Global Economy?

As with most changes in life, there will be positive and negative impacts on society as artificial intelligence continues to transform the world we live in. How that will balance out is anyone’s...

20 July 2024 10,062 3 View

Do you agree that at some point in our lives each of us will be a person with disabilities?

There is several approaches towards the analysis of legal status of people with disabilities (e.g. medical, social or human rights approach). One way to include people with disabilities in society...

17 July 2024 3,798 13 View

What are the best ways to automate my job and live without working?

I want to know the best methods to automate my job and live without working?

12 July 2024 5,993 2 View