What is the best positive control for ROS measurement with CellRox ?

23 May 2016 4,170 2 View

Is it possible to use too much lysis buffer?

We are using a mechanical pipetting machine to pipette lysed cells in buffer into a 96 well plate from a 24. The protocol asks for 100 uL of lysis buffer to be used in the 24 well plate, but this...

15 May 2016 7,043 6 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

The Role of Town Planners in CAV Use?

Colleagues and I are about to begin a project investigating the role of town planners and local authorities in establishing the introduction of connected and autonomous vehicles (CAVs). What...

21 February 2021 2,247 3 View

Is it appropriate to use AIDA model (Assessment of Identity Development in Adolescence) on a non-clinical sample?

I am researching emotion regulation and identity development in adolescence. I've come across several inventories for assessing identity development and it seems like the AIDA scale is the most...

16 February 2021 1,261 1 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

Cell culture compatible glue/sealant?

Hello We are looking for a glue /sealant to fix and secure some glass parts of a live cell imaging chamber. It has to be biologically inert because it may come in contact with the medium, and...

01 February 2021 6,886 3 View

EMOJI-POLL-3 (Emoji-Association): Can you join the survey?

https://docs.google.com/forms/d/e/1FAIpQLSfaaCWZZqMIhoz5yj_5dxggvb6-cISx3GUHssYUWZWKbpx3UA/viewform Dear colleagues! We ask you to participate in a series of surveys related to the scientific...

26 January 2021 5,969 3 View

Does anyone have a copy of the sTOFHLA?

Peppercorn books is out of business, and that the creator of the test has been dead for over 5 years. I am looking to use this tool to assess literacy in community dwelling seniors as part of my...

25 January 2021 4,061 2 View

(Scale request) Anyone has the Malay version of Eating Attitude Test-26/40 (EAT-26/40)?

Dear Researchers, My research team is conducting a eating disorder related research in Malaysia and we would like to use the Malay translated version of EAT26/40. I have tried to email the...

24 January 2021 8,925 2 View

Can Cas13a protein directly purify from PAGE gel, please suggest me the method?

I am willing to Cas13 in vitro. Can it be directly purified from PAGE gel, please suggest me the method.

19 January 2021 1,136 3 View

What aspects of communication skill improves significantly regarding to the Autism Spectrum Disorder using PECS?

Children with ASD need a clear and effective way to communicate to reduce frustration and replace challenging or unacceptable behaviours (Beukelman and Mirenda, 1998; Webb, 2000). Augmentative and...

19 January 2021 1,160 3 View