Hi. Im workin on EAE induction in C57BL/6 mice. Im gonna pepare MOG-adjuvant emulsion but i don’t know the storage conditions of it. Some papers suggest storage duration for severeal days but i may need to use it later. Is there anybody experiencing with the durability of MOG-adjuvant emulsion used for EAE induction in mice? How long can it be stored and what is the optimum temperature?
Hello!
Quite honestly, I've never stored my emulsions. I typically use mine within hours of making it. If the emulsion sits for too long it will eventually come out of solution. Plus I would seriously begin to question the disease inducing ability of the emulsion if it was stored for more than a few hours. I would recommend making up what you need for that day's injection (plus a little extra), and using that emulsion up that same day. Then when you need the emulsion again, you make it fresh.
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