Hi Gourab,

The only real disadvantage if you are using CBB R250 is the relatively high detection limit compared with silver staining (~100 ng of protein vs ~1 ng). Colloidal Coomassie or CBB G250 stain offers a lower detection limit, ~10 ng of protein, and is apparently more reproducible than R250. What are you trying to achieve by staining?

Mark

Thanks Mark for your valuable suggestion.Actually i am trying to develop a dye that can be comparable to the coomassie blue.

Sounds interesting, Gourab. In that case, I should add that CBB R250 staining can take a rather long time without using tricks like microwaving to speed it up. Staining in the dye itself works best if I allow it to go overnight at room temperature, followed by destaining for a few hours the next day (changing the destain every few hours), followed by a final wash with 5% acetic acid to remove residual background dye to really let the bands shine.