Hello everyone,
My aim is to isolate surface proteins from a parasitic nematode, however I have looked at protocols on line and most seem to be the same for 'cuticular' and 'surface' proteins, some protocols even talk about 'cuticular surface proteins'. I will be grateful if I could get a little help on this.
Cuticule is the most external layer of nematode tissue.
https://www.google.es/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&cad=rja&uact=8&ved=0ahUKEwiJ9uW-hJbYAhWY3oMKHTYtAAEQjRwIBw&url=http%3A%2F%2Fcal.vet.upenn.edu%2Fprojects%2Fmerial%2FNematodes%2Fnems_4a.htm&psig=AOvVaw1gojkNEXfM9AXuuLWOmWbo&ust=1513771517908990
You might consider looking at works by Roland Perry, he taught me at Ghent University as a Visiting Professor.
You will find useful information from this work;
Molecular and Physiological Basis of Nematode Survival
Hi Robert,
I think that both terms are interchangeable and mostly describe the same group of proteins. However, all cuticular proteins does not need to be in contact with external environment of nematode and you could use "surface protein" to specifically describe the protein in outer layer of cuticle.
There could also be a difference in isolation. Proteins in cuticle will be more difficult for isolation than proteins displayed on its surface.
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