Both nested clade analysis and phylogenetic tree are often used in phylogenetic studies. I want to know the difference between these 2 terminologies
I have sequenced a 7 kb part of frog mtDNA and have generated a bam file of the sequence. In IVG, I can visualize manually the per base read coverage but I need this information in an excel format...
05 June 2018 5,424 2 View
Can anyone explain me the details of the process enrichment PCR often used for sequencing purposes
10 November 2016 6,158 0 View
At the annealing step of PCR it is the oligonucleotide primers that binds to the single stranded template and initiates extension. But do the two single strands also anneal among themselves?
05 June 2016 3,807 3 View
I am trying to design primers for amplifying certain genes across the amphibian taxa. I know that the lower limit for designing primers is 18 bases for reasons of specificity. However, what could...
04 May 2016 6,148 6 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...
22 July 2024 9,761 5 View
Hello, I would like to ask which is the best terminology to use for describing a negative control in an experiment (drug testing in-vitro, viral infection): NC (negative control) or NTC? Thank you...
18 July 2024 4,183 2 View
We are trying to extract DNA from ant nests to amplify the 16S gene, but apparently, the extraction allows the passage of PCR inhibitors. Thank you for your comments
15 July 2024 9,314 2 View
Dear Researcher, I have used nested PCR to increase the specificity of Tetra Arms. Previously, I obtained results for another SNP using the same reaction conditions, and separate reactions...
14 July 2024 430 4 View
Dear colleagues I would like to know which Astigmata species I found in left cormorant nests. Could somebody help me with the identification?
31 May 2024 3,887 1 View
Additionally, could you suggest any related articles or resources on this topic?
07 May 2024 481 0 View
I am running a Nested PCR on blood DNA. Although the primers have worked successfully in previous publications, I am not getting a clear bands for sequencing. The first PCR reaction mix...
25 April 2024 4,661 3 View
For my thesis on the same HPV L1 region sequencing for detecting genotypes , I have used My09/11 & GP5+/6+ primers for amplification on Nested PCR, but I couldn't find sequences other than 16,...
19 April 2024 1,747 1 View
Can the acidity of the silicon hydroxyl group of zeolite, whether it is the terminal hydroxyl group or the silanol nest, catalyze the ethanol dehydrattion?
11 April 2024 1,235 0 View