Both nested clade analysis and phylogenetic tree are often used in phylogenetic studies. I want to know the difference between these 2 terminologies
I have sequenced a 7 kb part of frog mtDNA and have generated a bam file of the sequence. In IVG, I can visualize manually the per base read coverage but I need this information in an excel format...
05 June 2018 5,348 2 View
Can anyone explain me the details of the process enrichment PCR often used for sequencing purposes
10 November 2016 6,085 0 View
At the annealing step of PCR it is the oligonucleotide primers that binds to the single stranded template and initiates extension. But do the two single strands also anneal among themselves?
05 June 2016 3,737 3 View
I am trying to design primers for amplifying certain genes across the amphibian taxa. I know that the lower limit for designing primers is 18 bases for reasons of specificity. However, what could...
04 May 2016 6,060 6 View
Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...
02 March 2021 6,865 1 View
For my master thesis I am conducting a phylogenetic research and for this I'm using tools like MEGA, IQTREE, FigTree, etc to make a phylogenetic tree. I wanted to know if IQTREE integrates the...
14 February 2021 1,541 2 View
HI all, I have been working with daily diaries data ( 6 emotions of which 3 positive and 3 negative) were assessed for 30 days. These observations (1level data) are then nested within subjects...
13 February 2021 1,810 3 View
I have been working on a study trying to estimate body mass in mammals using a skeletal proxy in R. My dataset has high phylogenetic signal (Pagel's Lambda = 0.9) and I am interested in removing...
26 January 2021 6,997 3 View
Hi all, Considering that one has the whole genome sequence (annotated) of a bacterium and wants to know if it has the potential to cause human diseases, what steps would you recommend...
26 January 2021 8,732 3 View
Hello Researchers. I am conducting a study on the determination of WNV on mosquito samples. I run nested PCR. Bands were shown to almost all samples during carrying out gel electrophoresis. After...
17 January 2021 3,263 11 View
Hi I want to replicate a published article that uses a multilevel model (participants nested into groups). I want to see the sample size I need to achieve an 80% power. I saw the simr r package...
06 January 2021 2,984 6 View
I am doing semantic analysis of science and grey literature publications in the field of plant genetics and breeding. I have a standard dictionary but I am not sure if it captures the state of art...
31 December 2020 5,871 4 View
Im refering to Andy Field's example in his book Discovering statistics using SPSS, 3rd edition, page 728, where he gives an example of a data structure with level 1 variable is a memory recall...
26 December 2020 9,717 6 View
I have a regression equation in which I am curious if the amount of scatter around the regression line is driven by phylogeny. I know there is a lot of phylogenetic signal because Pascal's lambda...
22 December 2020 937 1 View