Debjyoti Dutta

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Questions related from Debjyoti Dutta

How to obtain per base read coverage of a bam file in excel format?

I have sequenced a 7 kb part of frog mtDNA and have generated a bam file of the sequence. In IVG, I can visualize manually the per base read coverage but I need this information in an excel format...

06 June 2018 5,387 2 View

What is the difference between nested clade analysis and phylogenetic tree?

Both nested clade analysis and phylogenetic tree are often used in phylogenetic studies. I want to know the difference between these 2 terminologies

11 November 2017 612 0 View

What is enrichment PCR?

Can anyone explain me the details of the process enrichment PCR often used for sequencing purposes

11 November 2016 6,129 0 View

Why don't single strands anneal among themselves during annealing step of PCR?

At the annealing step of PCR it is the oligonucleotide primers that binds to the single stranded template and initiates extension. But do the two single strands also anneal among themselves?

06 June 2016 3,783 3 View

What are the disadvantages of designing a very long primer (50 bases>)?

I am trying to design primers for amplifying certain genes across the amphibian taxa. I know that the lower limit for designing primers is 18 bases for reasons of specificity. However, what could...

05 May 2016 6,128 6 View