Now i do pharmacological research in fish so any one answer the question.
LD50 is the concentration causing 50% cell death (LD = lethal dose), although some people may have the opinion that LD50 should be reserved for in vivo studies only.
ED50 (or EC50) is the Dose (or Concentration) causing 50% of maximum effect for any measured biological effect of interest. This could include cytotoxicity (although in this case it is better to use LD50 in my opinion).
If you have an Excel sheet, you have to make a graph by plotting percent inhibition/ percent mortality on Y axis and concentration/ dose on X axis by using excel. Then you can calculate LD50 manually by using just a ruler or by an equation Y=mX+c. You can get the equation of your result by- place your mouse on curve> press right button> add trend line> options> show equation on chart. Then put Y=50 and calculate the value of X i. e. LD50 and so on.
To find the EC50 value you can use the enclosed equation to derive it, where Y is the observed value, Bottom is the lowest observed value, Top is the highest observed value, and the Hill coefficient gives the largest absolute value of the slope of the curve.
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