Dear Jia Jiet Lim!
Here is the explanation of CCK-8, with the molar mass of Calcein!
I hope it is enough detailed!
Sincerely Yours!
Dr. Bratko Filipič
E-mail: [email protected]
The Cell Counting Kit (CCK) is utilized for the fluorometric detection of living cell numbers. The amount of a fluorescent dye, calcein, produced from calcein-AM (3′,6′-Di(O-acetyl)-2′,7′ -bis[N, tetra acetoxymethyl N-bis-(carboxymethyl) amino met tetra acetoxymethyl ester) by esterases in cells is directly proportional to the number of viable cells in a culture medium. Since calcein-AM is highly lypohilic because of the acetoxymethy groups in the molecule, it can rapidly permeate into the cytoplasm through the cell membrane. The CCK assay does not require any radioisotopes (such as in the [3H]- thymidine incorporated assay) or a solubilization procedure (such as in the MTT assay). Therefore, it allows the users to obtain highly reproducible and accurate cell proliferation assay results.
Calcein
Chemical formula: C30H26N2O13
Molar mass: 622.55 g/mol
pg/mL X 1.000 to convert pg in 1.000 mL and so divide per MW of CCK we get pmol/L.
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