Please suggest the concentration of stock and working solution of DCFDA and the solvent needed for making the solutions!
DCFDA ( Stock 10 mM ) in 1X PBS mixture should be added in such a
way that the working conc. becomes 20µM.
The dielectric constant of my samples show a sudden drop at around 60degree, whereas they are heat stable and shows no change in phase in DSC TGA. What can be the possible reasons for such change?
06 July 2018 3,750 4 View
How can naturally formed colloids/ nanoparticles be useful in remediation of environmental problems and degradation?
06 July 2018 9,013 6 View
Hi! My sample is sparingly soluble in both polar and non polar solvent, then kindly suggest the best technique that I can follow to analyze the antimicrobial efficacy of my sample!
05 June 2018 7,425 1 View
I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...
03 March 2021 7,540 4 View
I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...
02 March 2021 4,443 2 View
We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...
02 March 2021 3,713 2 View
I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...
02 March 2021 1,404 6 View
I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.
01 March 2021 4,306 1 View
I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...
01 March 2021 9,525 4 View
Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?
01 March 2021 9,447 3 View
28 February 2021 8,466 2 View
The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...
28 February 2021 2,955 2 View
there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...
27 February 2021 2,609 3 View