I have isolated some fungi in Rye A, media from infected leaf of potato and tomato.
While staining the infected portion of leaf, that showing typical P.infestant symptoms I observed typical lemon shape Phytophthora spores.
But during isolation process, I got number of fungi with different morphology, with various color.
Please could someone suggest me what should be the colony morphology of P.infestans?
Phytopthora es dificil de aislar por eso se obtienen colonias de microorganismos contaminantes. Es necesario utilizar tejido adyacente a la zona de avance de la lesión. Las colonias de este cromista son blancas con micelio esponjoso y sin septas
. Hay que usar un coctel de antibióticos en el medio para evitar contaminaciones
Generally, the mycellium (in culture) should be white & fluffy. In case of A2 mating type, it produces lumpy appearance.
Remember one thing, initial colonies (from inoculation with lesion pieces) are frequently infected by other fungi & bacteria. However, inoculation with diluted sporangial suspension produces more pure colony of the fungus.
Once fluffy mycelium is visible firstly, you can transfer some to antibiotic plates (e.g., PARP or RVN) and then subculture to ensure the purity of the isolation.
You can follow the below two papers with two different techniques:
http://www.sciencedirect.com/science/article/pii/S0269915X00800968
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3464107/
Best Wishes,
Thank you Somnath for the kind information. I will be better if some pictures will available....
OK. Follow the below link. You can get all the images regarding leaf symptoms, colony morphology, structure of sporangia, sporangiophore, oogonia & anthredium & many other characteristics related to P. infestans (causing late blight of potato). Although it is not in Rya A, but the images will be the almost same.
Best,
http://www.q-bank.eu/Fungi/BioloMICS.aspx?Table=Phytophthora%20species%20ONLINE&Rec=588&Fields=All
http://www.q-bank.eu/Fungi/BioloMICS.aspx?Table=Phytophthora%20species%20ONLINE&Rec=588&Fields=All
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