file:///C:/Users/pc7/Downloads/Exploring%20opportunities%20for%20development%20of%20bioinsecticide%20to%20combat%20harmful%20organisms%20on%20the%20tobacco.pdf Determining nicotine in aqueous extract of tobacco is possible. This article can help or guide you. Sorry in Bulgarian, you will need a translation.

http://cropscience-bg.org/page/en-topmenu/home.php  This is the journal's website. There may be other relevant articles.

The term ‘‘tobacco constituent’’ is defined as a substance naturally present in tobacco. The term ‘‘tobacco ingredient’’ is defined as a substance (except water) that is added to tobacco during the manufacturing process and having a specific function on the final tobacco product. Tobacco ingredients are classified as flavours and additives. In 1960, a little over 200 chemical constituents had been identified in tobacco leaf of all types and less than 450 had been reported in smoke. Today, approximately 3000 have been identified and characterized in tobacco leaf and some 4000 in smoke. Estimates are that the total number of chemical constituents in leaf exceeds 4000 and there are over 6000 in tobacco smoke. Mixture tobacco for cigarette consist flue-cured tobacco- virginia, air-cured tobacco-burley, sun-cured tobacco. reconstituted tobacco (RECON) and expanded rib which differ according to the chemical composition. The smoke composition is depend from composition of tobacco mixture.

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