Molecular techniques for detection the resistance gene in microbial strain.
One way is to select for resistance by growing a large inoculum of the parent susceptible strain on agar containing the drug at a concentration above that required to inhibit growth in order to obtain resistant colonies. Then sequence the genomes of several of these resistant colonies and compare the genomes to the parental genome to see which genes have mutated. Sometimes, resistance will be due to a mutation in the gene for the target , sometimes not.
One way is to select for resistance by growing a large inoculum of the parent susceptible strain on agar containing the drug at a concentration above that required to inhibit growth in order to obtain resistant colonies. Then sequence the genomes of several of these resistant colonies and compare the genomes to the parental genome to see which genes have mutated. Sometimes, resistance will be due to a mutation in the gene for the target , sometimes not.
What is your point exactly?
If you want see your bacteria have or have not a resistance gene so use PCR.
The way that dear Adam B Shapiro mentioned is very perfect way when you have a bacteria that include susceptible and resistance strains without present of any resistant gene.
That is interesting you know that sometimes bacteria have resistance gene but phenotypically they are susceptible i.e strains of staphylococcus aureus that have mec gene but they are susceptible to meticilin and it is because of mutation.
Hi,
guess both ways have its advantages. I would also propose to use the PCR as you cannot culture all bacteria/ you need various different culture condiutions. Be aware that there are several different resistance cassettes even for the same resistance. Another approach could be whole genome sequencing. The beauty here is that you get also genetic motifs you might not expect/ your PCR would not cover. In the end it depends on the technical capabilities in the lab and budgets
Best Sven
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