29 July 2025 0 5K Report

I am working on rat brain histopathology and need to clearly differentiate the CA1 and CA3 subregions of the hippocampus for neuronal loss analysis. I am currently using paraffin-embedded brain sections (5 µm) and staining with H&E, but I often face difficulties:

  • The boundaries between CA1 and CA3 are not always clear.
  • Tissue shrinkage during processing makes it hard to match the sections with stereotaxic coordinates.

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