As I used hexane for FAME dilution but it is not properly getting diluted but when dissolved in chloroform it got dissolved.
Fatty acid methyl ester (FAME) is a relatively higher polar chemical compared to a hydrocarbon(hexane). This is the reason for better dissolution of FAME in chloroform. So you need a polar compound to make a dilute solution. You may try ethyl acetate as a solvent.
I have tried to give you a general guideline (polarity ) about the choice of solvent. It will also depend on the purpose of your experimentation.
chloroform or toluene. I used both of GC MS and FID and they work just fine
The chloroform is best solvents for fatty acids and poly aster compounds
@alaa but for GC MS analysis the sample must be diluted in the hexane only said by the GCMS analyst that why i did nt know what to do
I think due to the presence of some polar compounds, it not dissolved in hexane. You should put NH4OH on that (FAME). Try this with very minute sample.
Theses papers might be helpful:
- Preparation of fatty acid methyl esters for gas-liquid chromatography.
- Development in lipid anaylsis: some new extraction techniques and in situ transesterification.
- Preparation of Fatty Acid Methyl Esters for Gas-Chromatographic Analysis of Marine Lipids: Insight Studies.
- A direct method for fatty acid methyl ester synthesis: Application to wet meat tissues, oils, and feedstuffs
- Preparation of fatty acid methyl esters for gas chromatographic analysis of lipids
in biological materials.
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