What is the best simple method to purify 2-aminoethyl methacrylate hydrochloride monomer stabilized with phenothiazine inhibitor?

20 December 2022 0 10K Report

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Can sodium dithionite be used for reduction of nitro to amine group? Is this better than Fe powder which needs column separation?

I want to reduce the nitro to the amine group. But with Fe powder, requires column separation. I am interested in direct reductive products without the use of columns. Can this be achieved by...

04 August 2021 8,980 2 View

Alternative for DEAD in mitsunobu reaction?

I am preparing an ether derivative from two alcohol reactants. Can it be possible by DMAP or any base catalysed reaction.

02 August 2021 9,209 4 View

Work up of ester?

What is the detailed procedure for work up in ester synthesis from acid chloride and alcohol? What organic solvent to use for extraction?

18 January 2021 8,807 6 View

How to dry the pyridine?

How to make the pyridine dry? Preferably with NaOH drying agent. I want the detailed procedure

14 January 2021 3,262 4 View

How to dry THF?

I want to know the detailed procedure for drying it. I am more interested to use KOH for drying. I want the procedure from starting.

14 January 2021 2,976 7 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

How do you determine the Ki of an inhibitor for an enzyme which also undergoes substrate inhibition?

There are three factors involved in the activity, the substrate acting as a substrate, the substrate acting as an inhibitor and another inhibitor for which you want to measure the Ki.

08 July 2024 4,988 1 View

How to incease the yiald of recombinant protein expression ?

Hello esteemed scientists, I am endeavoring to increase the yield of my recombinant protein. It is a 120 kDa protein tagged with MBP and features three intrinsically disordered regions with pI...

12 June 2024 9,616 8 View

Can you explain the potential toxicity and overdosemanagement of commonly used antiplatelet drugs?

Antiplatelet Therapy: Mechanisms, Indications, Administration Can you explain the potential toxicity and overdosemanagement of commonly used antiplatelet drugs?

05 June 2024 6,447 1 View

How to prepare 0.28 U/ml working solution of Acetylcholinesterase inhibition assay for anti-Alzheimer activity?

I have purchased Acetylcholiesterase from Electrophus electricus (electric eel), C3389-2KU, following details are written on the enzyme vial. Type-VI S lyophilized powder 200-1000 Units/mg...

31 May 2024 6,444 1 View

Can anyone interpret why the absorbance in alpha amylase assay get increased with increased standard (inhibitor) concentration?

Can anyone interpret why the absorbance (DNS assay method) in alpha amylase assay get increased with increased standard (inhibitor) concentration? it should be decreased with increased standard or...

26 May 2024 4,753 2 View

Should the target miRNA level decrease after the miRNA inhibitor transfection when checked with qPCR??

I have a question regarding target miRNA expression after transfection with antisense miRNA (miRNA inhibitor). I understand that the expression of the target miRNA will increase after mimic...

20 May 2024 5,920 4 View

Why are protease inhibitors necessary in denaturing conditions?

I know that conventional wisdom dictates that protease inhibitors should be added to lysis buffers when extracting protein to prevent sample degradation. That said, why are such inhibitors...

14 May 2024 2,145 2 View

Is trying to position an activator compound in the pocket where the inhibitor binds a targeted approach?

If a 3D protein is obtained with the inhibitor complex, its wild-type size may change completely. In this case, it seems correct to investigate the binding mode with the inhibitor molecule. If the...

26 April 2024 3,509 5 View

How to modelling MD of Covalent Inhibitor?

I'm designing a covalent inhibitor on an in silico study. Should I use the "pre-reaction" non-covalent ligand-protein complex or covalently bonded one on the Molecular Dynamic simulation input? I...

20 April 2024 5,662 2 View