What is the best set-up for hollow fibre membrane filter module to filter microspecies produced from running anaerobic fermentation medium?

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To avaoid yeast contamination in algae cultivation, what strategy would be effective? I don't think lower pH because yeast grows optimally 4.5-5.5 ?

strategy would be cost effective and applicable to industrial scale

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what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

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What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

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How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

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Where can I find life cycle inventory or assessment of non woven materials?

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Dapi fluorescence protocol for colon cell lines ?

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What is the exact mechanism of the antimicrobial effect of local anesthetics?

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Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

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Advice on growing lactobacillus species in the lab?

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Juergen Wiegel

We had not so much success with the horizontal filter approach due to slime etc. production. However we had great success using the Amicon 1 million cutoff (autoclavable also much cheaper than the filter, much more robust, if you need more flow you can run several of the tubes in parallel fashion or buy the larger sizes.The key point for success was putting the filter in so that you could reverse every three daysor so the flow through the hollow fiver tubes without disconnecting, By this way we were able to run fermentations for more than2 month without interruptions for producing acetic acid under cell recycling modus: for drawings and description see. L. G. Ljungdahl, L. H. Carreira, R. J. Garrison, N. E. Rabek, L. F. Gunter, and J. Wiegel. (1986). CMA-Manufacture (II): Improved bacterial strain for acetate production. Final Report to Federal Highway Administration (FHWA/RD-86/117-22161). National Technical Information Service, Springfield, VA..J. Wiegel, L. H. Carreira, R. Garrison, N. E. Rabek, and L. G. Ljungdahl. 1991. Calcium magnesium acetate (CMA) Manufacture from glucose by fermentation with thermophilic homoacetogenic bacteria. Chapter 16 In: "Calcium Magnesium Acetate", Eds., D. L. Wise, Y. A. Levendis and M. Metghalchi. pp. 359-418. Elsevier Science Publisher, Amsterdam-New York.

Anil Kumar Patel

Thank you Prof. Wiegel for your valuable informations and sharing experinece with me. It will be a great help to solve related problems with my new approach of ongoing experiments.

Vasileios Diamantis

Anil hi, You need a lab-scale crossflow ultrafiltration membrane (eg some 0.5 m) and a peristaltic pump. The latter recycles on demand the fermentation medium through the membrane module while you receive the permeate . The overall setup is very simple to construct.