What is the best set-up for hollow fibre membrane filter module to filter microspecies produced from running anaerobic fermentation medium?

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Vasileios Diamantis

Is it for laboratory purposes or for full-scale design?

Anil Kumar Patel

For lab scale

Juergen Wiegel

We had not so much success with the horizontal filter approach due to slime etc. production. However we had great success using the Amicon 1 million cutoff (autoclavable also much cheaper than the filter, much more robust, if you need more flow you can run several of the tubes in parallel fashion or buy the larger sizes.The key point for success was putting the filter in so that you could reverse every three daysor so the flow through the hollow fiver tubes without disconnecting, By this way we were able to run fermentations for more than2 month without interruptions for producing acetic acid under cell recycling modus: for drawings and description see. L. G. Ljungdahl, L. H. Carreira, R. J. Garrison, N. E. Rabek, L. F. Gunter, and J. Wiegel. (1986). CMA-Manufacture (II): Improved bacterial strain for acetate production. Final Report to Federal Highway Administration (FHWA/RD-86/117-22161). National Technical Information Service, Springfield, VA..J. Wiegel, L. H. Carreira, R. Garrison, N. E. Rabek, and L. G. Ljungdahl. 1991. Calcium magnesium acetate (CMA) Manufacture from glucose by fermentation with thermophilic homoacetogenic bacteria. Chapter 16 In: "Calcium Magnesium Acetate", Eds., D. L. Wise, Y. A. Levendis and M. Metghalchi. pp. 359-418. Elsevier Science Publisher, Amsterdam-New York.

Thank you Prof. Wiegel for your valuable informations and sharing experinece with me. It will be a great help to solve related problems with my new approach of ongoing experiments.

Anil hi, You need a lab-scale crossflow ultrafiltration membrane (eg some 0.5 m) and a peristaltic pump. The latter recycles on demand the fermentation medium through the membrane module while you receive the permeate . The overall setup is very simple to construct.