A possible way to do so. The reaction mixture (5 ml) consisted of 0.2 ml of egg albumin, 2.8 ml of phosphate-saline buffer and 2 ml of extract with different concentrations of your plants, so that the final concentration was 31.25, 62,5, 125, 250, 500, 1000 mg/ml. the Same volume of double-distilled water served as control. The mixture is incubated at a temperature 37 ± 2 °C for 15 minutes and then heated at 70 °C for 5 minutes. After cooling was measured their absorbance at 660 nm using a control sample. Diclofenac sodium at the final concentration (78,125, 156,25, 312,5, 625, 1250, 2500 mg/ml) was used as reference drug. The percentage inhibition of protein denaturation was calculated by the formula: % Inhibition = 100 × [VT/ VC — 1] where, Vt = absorbance of test sample, Vc = absorbance of control.
Carrageenan test is best method for assessment the ani inflammatoy activity
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