Are there any valid protocols? We can't propagate via stem cutting and seed.
Dear Saeid
If not possible in stem cutting and seed propagation.
please try for in vitro propagation (biotechnology tools)
1) i want to know about seed and cutting treatments
2) is there any protocol for in vitro propagation
seeds are treated in ho twater, drained and washed several times sown in cold trays. hot water treatment is critical. Also root cuttings, taken in winter are possible, but require sampling sections near a finger size.
tanks a lot, the majority of our collected seeds don't seem fertile, is it correct ?
Stem cuttings are surface cleaned with water and a few drops of a mild household detergent, and then cut into 20 cm in length and 1.5 to 2.0 cm diameter are made using a sharp knife, the basal cut was made just under a node.
Cuttings were divided in three groups of treatments:
i) with leaves pruned to 1/3 of their area,
ii) ii) with leaves totally removed and,
iii) iii) with leaves intact.
All cuttings are immersing for 5 minutes in a 6% (w/v) solution of Bavistin in water and allow to air dry. Cuttings were then basally quick dipped up to 2.5 cm deep in different water solutions of IBA/IAA/ NAA at different concentrations. The cuttings are planted immediately after treatment, 4 cm deep, into one of two rooting media in soil mixture and compost (1:1). The observation is taking after three months record on percentage of rooting, number and length of roots as well as number and height of sprouts per root cutting.
Rhus coriaria seeds were found to show double dormancy; see https://www.researchgate.net/publication/258125975_Post-_fire_regeneration_of_hardseeded_plants_ecophysiology_of_seed_germination.
You may want to follow the procedures described in that paper.
Conference Paper Post-fire regeneration of hardseeded plants: Ecophysiology o...
dear Thanos
hi
I can't get this paper . can you please send me a copy of it?
thanks a lot
when we cut stem of sumach, we can see cohesive and very aromatic liquid. do you know its components? any references?
dear Bongi
i do your recommendation about root cutting and seed, but after about 3 months i can't see anything. what can be wrong?
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