What is the best method for RNA extraction from blood by trizol?

10 October 2018 1 8K Report

I have extracted RNA from blood samples by trizol method but sometimes it give very good quality but some times degraded. Can somebody explain the point to consider while isolating RNA from blood by using trizol

Zx Chong

I think one of the important points is the cell counts before you store the RNA with Trizol. If the cell count is low before addition of Trizol, it might give low RNA yield. Besides, other factors could be related to the sample storage condition and RNA is quite fragile compared to DNA, thus it tends to degrade during storage or while during RNA extraction.. Sometimes I face similar problem and what I can say is that patience is an important key and just pray hard!

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