Molecular detection techniques.

Thank you adekunle for the references, God bless

Hello, You can concentrate your samples by centrifugal and filtration methods, following with Quantitative PCR techniques to identify organisms at the level of species. qPCR let you quantify the density of organisms in a fixed volume dilution

Thanks Guy. but wont i need a control sample for the QPCR.

I dont know what kind of parasite you are working on. But if same my case, Please use net to get eggs, eggs will attach to net  and get oncomiracidia after incubation net with eggs attached. 

See my paper and it may give you some ideas.

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0108801