What is the best etching solution for hydrogen capped with silicon?

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Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I use different force field for protein and ligand?

Hello, Dear! Generally, I used CHARMM36 + cgenff force field for simulaiton. But,, due to certain parts of lignad, force field cannot be generated using Cgenff. Can I use different force field...

10 July 2024 9,513 3 View

Ways to mitigate stimulation electrodes coming off?

I have a cathode electrode (5 x 5cm) on the femoral triangle and an anode electrode (5 x 10cm) on the gluteal fold. We are doing repeated high-intensity cycling followed by repeated Wingates. We...

07 July 2024 833 1 View

How to solve Gaussian Convergence failure?

To whom it may concern, I'm working on optimizing the molecule using gaussian 16. However, it is suffering from the problem of convergence failure. I attempted optimization several times, but...

30 May 2024 6,373 3 View

Setting chlorine injection concentration??

I want to experiment with microbial control through chlorine disinfection. What I have on hand is 5% sodium hypochlorite solution. I want to set the chlorine injection concentration to 1, 3, 5,...

01 May 2024 3,741 0 View

What is the mechanism of ionization of transition metal?

can anyone give me advise about charge transfer transition of transition metal and rare earth element? and usually people see the FL and absorption, emission spectra of those materials in glass,...

08 March 2024 7,916 1 View

How to dissociate clumping cell?

H1781 cells are shown to tend to clump each other. H1781 is adherent cell and detached by using trypsin-EDTA. however, over-digestion using trypsin might provoke cell clumping. they seemed like...

03 March 2024 7,757 4 View

What is wrong with the mitochondria isolation protocol?

Hello I am currently trying to set up a new protocol. Our lab is planning to perform study about mitochondria, and we still couldn't set up a proper protocol. The kit we used is from abcam...

05 November 2023 9,681 0 View

Why have most research studies related to crack branching been conducted only on amorphous materials?

I've been studying the crack branching and dynamic fracture of brittle materials, and I just noticed that most of the experiments are conducted using amorphous materials like PMMA. is there...

26 October 2023 7,536 0 View

M-wave acquisition Matlab script?

I would like to acquire EMG, torque/angle (from isokinetic dynamometer), and stimulation data via Matlab. I have a chassis system that is connected to my computer with Matlab. When the stimulation...

18 July 2023 7,425 2 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View

In the presence of VERY high concentration (1000 ppm) of silicon, the adsorption isotherms of arsenic are showing anomalous trends, why so?

I conducted an adsorption experiment of arsenic on soil in the presence of different doses of silicon as competing ions to see the effect of silicon on arsenic adsorption and desorption. I took 5...

03 August 2024 6,500 3 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View

What is the ionic radius of Hydrogen anion? And how to know the exact ionic radii of the elements?

I'm trying to calculate the tolerance factors of the hydride perovskite and the value calculated by me and the reported values are contradictory. Need more clarity in using the correct ionic radii...

01 August 2024 2,614 1 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

How to make the concentration measurements for the hydrogen absorption/desorption in the metal hydride?

Hello Everyone! I am trying to create a metal hydride system where I am trying to create a temperature control with the emphasis on measuring the concentration of the hydrogen with the hydrogen...

29 July 2024 7,577 2 View