Why HRLDAS need to combine snow and rain as Precipitation? Why this need hourly interpolation of solar radiation?

Dear coworkers, my questions are about HRLDAS. I am runing HRLDAS with GLDAS-based forcing data. For running offline LSM such HRLDAS, we know we need atmospheric forcing and initial data. In...

28 March 2020 8,726 3 View

Can anyone recommend a good caspase-3 antibody?

I'm looking for an antibody that can detect both procaspase-3 (>30kDa) and active caspase-3(>15kDa) using western blot analysis. Did you test this experimentally? Did you get good results using a...

20 January 2020 112 3 View

How to treat poly(I:C) (polyIC, poly-IC, polyI:C) to NS-SV-AC or other cell line?

Hi, I'm experimenting to check the effect of treating polyIC (poly(I:C), polyIC, poly-IC, polyI:C) to NS-SV-AC cell lines. But I haven't been witnessing a significant cell death after treating...

23 July 2019 1,690 1 View

Is impacting the only option for experimental modal analysis of structure with free-free boundary condition?

For the modal testing of free boundary condition structure, such as a steel beam put on the bungee cords, is it possible to apply force using the actuator, like modal shaker? I guess it is...

24 June 2019 3,806 3 View

How can I estimate the purity of a recombinant protein after purification process using size-exclusion chromatography (no standard product exists)?

I produced a recombinant chimeric protein from CHO cells and purified it using size-exclusion chromatography(SEC). Thankfully, I could get a clear peak for my target protein and separate it from...

23 April 2019 4,383 12 View

How to decide sample size for validation?

I have data having over 20000 rows. 1) I am going to use a tool to test how many error rates this tool will calculate in this data. 2) And then, I will randomly choose some data and check how...

24 March 2019 7,656 4 View

Do i have to include all the hydrogen atoms for atomic charge calculations using GROMOS force field?

Dear all, I know that GROMOS96 force filed is united atom. When calculating the atomic charge using the GROMOS96 force field, are the value calculated considering all hydrogen atom in the ligand?

16 January 2019 7,978 1 View

How can i use EMBL pride inspector?

Hellow everyone. I am supposed to use EMBL pride proteomics documents. But I don't know how to use EMBL pride inspector program. even there is no file to introduce inspector program. Up to now, I...

29 May 2018 4,754 2 View

Are there any tries or results about carbon nanotubes film prepared through vacuum thermal evaporation?

I am searching some tries related to it. But most of the search results are about a synthesis of the carbon materials itself via chemical vapor deposition (CVD).The term 'CVD' contains 'vapor' so...

19 June 2017 3,463 2 View

Looking for a polymer with specific properties

Could anyone suggest a polymer having following properties? 1. It should be easily soluble in water at 20 degree C 2. It should NOT be soluble in ethanol 3. It's melting point must be well above...

11 June 2017 3,547 5 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Which is the most efficient etching solution for nickel based alloys?

Which solution reveals the microstructure and features of nickel-based alloys?

02 March 2021 2,221 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

How to model Supercritical water gasification (SCWG) process in Homer software?

Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?

25 February 2021 4,075 1 View

How can I selectively dry etch Ti on a polymer substrate?

24 February 2021 5,378 1 View

How the Ground Plane implantation is performed without damaging (and w/o varying the undoped nature) the top Si (8-10 nm) in FDSOI technology ?

It is a general practice to introduce Ground Plane in FDSOI devices under the BoX. The Ground Planes are introduced by Ion Implantation through Top Si layer and Buried Oxide. I wanted to know that...

24 February 2021 5,974 4 View