Dear Terrens,

FM 4-64 stained the inner membrane preferentially. For details see the following paper.

Mol Microbiol. 1999 Jun;32(6):1166-72.

Visualization of membrane domains in Escherichia coli.

Fishov I1, Woldringh CL.

http://www.ncbi.nlm.nih.gov/pubmed/10383758

Abstract

Bacterial membrane and nucleoids were stained concurrently by the lipophilic styryl dye FM 4-64 [N-(3-triethylammoniumpropyl)-4-(6-(4-(diethylamino)phenyl) hexatrienyl)pyridinium dibromide] and 4',6-diamidino-2-phenylindole (DAPI), respectively, and studied using fluorescence microscopy imaging. Observation of plasmolysed cells indicated that FM 4-64 stained the inner membrane preferentially. In live Escherichia coli pbpB cells and filaments, prepared on wet agar slabs, an FM 4-64 staining pattern developed in the form of dark bands. In dividing cells, the bands occurred mainly at the constriction sites and, in filaments, between partitioning nucleoids. The FM 4-64 pattern of dark bands in filaments was abolished after inhibiting protein synthesis with chloramphenicol. It is proposed that the staining patterns reflect putative membrane domains formed by DNA-membrane interactions and have functional implications in cell division.

Hoping this will be helpful,

Rafik

Dear Terrens Saaki,

 [N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide)] also called as DAPI is the best dye for  staining E.Coli inner membrane. 

Hi Poorna,

I know DAPI stained the nucloid, so the DNA. I also use this to stain the DNA and not the membrane? Are you sure its acronym is DAPI?

Dear Terrnes,

In my previous message, I sent you a paper indicating the use of this reagent. (DAPI) .

Rafik

Dear Rafik, I think you might have misread. When they state that:

"membrane and nucleoids were stained concurrently by the lipophilic styryl dye FM 4-64 [N-(3-triethylammoniumpropyl)-4-(6-(4-(diethylamino)phenyl) hexatrienyl)pyridinium dibromide] and 4',6-diamidino-2-phenylindole (DAPI), respectivel" they mean FM 4-64 forr membranes and DAPI for DNA... DAPI is indeed a classical DNA dye.

From the full paper "The fluorescence from live, FM 4-64-stained E. coli ... was restricted to the envelope alone".

Yoram

I assume you mean Blood serum and blood plasma. Assuming this is correct it really depends what you are looking for. Blood plasma contain some proteins that are absent in blood serum (due to coagulation) and therefore is likely to give simpler gels. Plasma  contains more proteins and clotting could be an issue.

What is your 'scientific question'?

Yoram