I'm still on a verge of looking the best assay of determining the antioxidant activity of my plant material which would be pseudobulbs of Philippine Ground Orchid (Spathoglottis plicata Blume)
Antioxidants are compounds capable to either delay or inhibit the oxidation processes which occur under the influence of atmospheric oxygen or reactive oxygen species. They are used for the stabilization of polymeric products, of petrochemicals, foodstuffs, cosmetics and pharmaceuticals.
Antioxidants are involved in the defense mechanism of the organism against the pathologies associated to the attack of free radicals.
Endogenous antioxidants are enzymes, like superoxide dismutase, catalase, glutathione peroxidase or nonenzymatic compounds, such as uric acid, bilirubin, albumin, metallothioneins. When endogenous factors cannot ensure a rigurous control and a complete protection of the organism against the reactive oxygen species, the need for exogenous antioxidants arises, as nutritional supplements or pharmaceutical products, which contain as active principle an antioxidant compound. Amongst the most important exogenous antioxidants, vitamin E, vitamin C, β-carotene, vitamin E, flavonoids, mineral Se are well known, but also vitamin D and vitamin K3.
Exogenous antioxidants can derive from natural sources (vitamins, flavonoids, anthocyanins, some mineral compounds), but can also be synthetic compounds, like butylhydroxyanisole, butylhydroxytoluene, gallates, etc [1].
There is an increasing interest in antioxidants, particularly in those intended to prevent the presumed deleterious effects of free radicals in the human body, as well as the deterioration of fats and other constituents of foodstuffs [2].