What is the best and easy technique to determine the antibacterial activity of inflammable latex of babool (a medicinal plant)?
Dear Dr Ajِeet Singh,
MIC
minimum inhibitory concentration (MIC) is the lowest concentration of an antimicrobial (like an antifungal, antibiotic or bacteriostatic) drug that will inhibit the visible growth of a microorganism after overnight incubation.
You can use agar well diffusion or agar well dilution methods.
Thanks sir
Perform following steps:
1. Dilute the latex at different concentrations in distilled water aseptically.
2.Perform Disc diffusion test as per standard methods (Buer et al).
3. Compare the result with standard antibacterials.
(These will be supplied by the manufacturer).
You may read/follow the following related article.
Article A COMPARATIVE STUDY OF EXTRACT OF SUCCULENT LEAVES OF LIVING...
Thanks.
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