I am working on the HPLC test of important plant hormones Gibberellin, Cytokinin and Auxin concentration in leaves. I have the samples of leaves but before this as you know standardization is recommended. I am new to this so I am looking for what will be the area at standard concentrations. And what will be the recommended run time.
Thank you so much
Just run the standard first, (follow the protocol) actually the time is according to the follow rate of solvent per minute.
I would recommend generating standard curves for each analyte of interest first. This will provide you with the retention times and limits of detection for each using your particular equipment. The run time will depend on several factors specific to your setup and research needs (mobile and stationary phases, flow rate, flow path length, detection method, resolution needed, etc.) and must be determined empirically. Literature from Karin Ljung and Ondrej Novak will provide you with much of the information you will likely need.
Best wishes!
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