What could be the reason I do not see knockdown at Protein level?

I am struggling with western blot since long time. Protein of interest is SATB1. I see very good knockdown on mRNA levels at 96 h. After knockdown, some cell lines start dying at 72 h while in...

02 March 2018 971 8 View

In a Denaturing PAGE (SDS), if the protein sample is degraded then do we see a smear or several bands near the protein of interest on western blot?

31 December 2014 9,964 11 View

What should be the buffer composition and other parameters to be considered for Annexin V staining to work?

I tried Annexi V staining coupled with FITC flouroscent stain and analysed it by flow cytometry. I tried these two buffer compositions: Buffer1: 10 mM HEPES/NaOH, (pH 7.4), 150 mM NaCl, 5 mM KCl,...

10 November 2014 7,947 7 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Centrality measures computation in Biblioshiny/ Vosviewer?

26 February 2021 7,279 2 View

Which detection method is better for an ELISA-based assay: colorimetric or fluorometric?

Hello, I am planning to perform an ELISA-based assay to quantify the percentage of genomic methylation (5-methylcytosine) in human embryonic stem cells. I've been searching for some kits and I...

16 February 2021 8,389 3 View

How can I quantify the color of a solution?

I know colorimetry has been the standard way by way of measuring the concentration of a particular compound (solute) in a colored solution (solvent or measure quantities of a particular compound...

08 February 2021 2,673 1 View

Add text at the beginning of a specific reference in Latex?

Hey researchers! The journal I'm submitting to wants datasets to be listed in the reference as [dataset] Authors; Year; Dataset title; Data repository ; Version; Persistent identifier (e.g....

18 November 2020 2,681 5 View

How do you include the literature in reference list that is not cited in the text?

Hi, as I know you should not cite the authors if you already include them in the sentence. But if I do not cite the study then it will not show in the reference list (I am using Zotero). How do I...

25 October 2020 9,203 3 View

How to convert color scales? Gardner to Lovibond to Apha to Ph. Eur ?

Hi there, Is it possible to convert between color scales like Lovibond, Gardner, Apha or the solutions mentioned in Ph. Eur ? I found descriptions of spectophotometers where e.g. EBC and SRM were...

07 October 2020 5,045 1 View

Can anybody recommend a suitable and simple assay for measuring total salicylic acid content of foodstuffs?

Looking for a simple assay, i.e. colorimetric, to determine the SA content of food (if one exists). Most methods in literature are HPLC-based and ideally want to avoid this.

09 September 2020 8,927 3 View

Extraction from soil for FIA analysis ?

Hi everyone, i would like to extract some elements and molecule from soil for an Continuous-Flow Analysis (Futura, AMS Alliance) but for these analysis I only have very little material (about...

26 July 2020 8,480 3 View

Why a colorimetric assay is usually not in green?

Various colorimetric assays we use are blue/purple/pink/red but not in green shades. Is there any particular reason for this?

30 May 2020 5,834 1 View