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I am struggling with western blot since long time. Protein of interest is SATB1. I see very good knockdown on mRNA levels at 96 h. After knockdown, some cell lines start dying at 72 h while in...
02 March 2018 1,032 8 View
I forgot to add protease inhibitor cocktail before I lysed my tumor tissue samples for protein extraction. For 30 µg of protein I needed an exposure time of 2 seconds for the Actin protein as the...
31 December 2014 10,267 11 View
I tried Annexi V staining coupled with FITC flouroscent stain and analysed it by flow cytometry. I tried these two buffer compositions: Buffer1: 10 mM HEPES/NaOH, (pH 7.4), 150 mM NaCl, 5 mM KCl,...
10 November 2014 8,017 7 View
Dear all, One of the key points in TSAD (Time Series Anomaly Detection) when using a sliding window is the size of the window. For (quasi)-periodic time series, methods such as AutoPeriod,...
13 May 2024 920 3 View
HEK Blue is a commercially available cell line with secreted embryonic alkaline phosphatase (SEAP) reporter. Readout of SEAP production is by production of blue colour in the detection medium,...
24 March 2024 6,931 0 View
Hej everyone, I'm working with humic substances in soil amendments. Last year, I outsourced measurements for fulvic and humic acids to a lab. After months, I sent freshly made samples to a...
05 February 2024 8,964 3 View
Several morphological characteristics (adaptative or not) could be associated with the process of adaptation to a specific environment or microhabitat. For example, several troglobization...
25 December 2023 5,431 3 View
I would like to know if there are bibliographic references on this subject, or also to know what are the maximum values of Ge and In that can be in a sphalerite.
05 October 2023 8,051 0 View
Here you are a message from an editor : ‘’ A preliminary quick review of your paper has made it clear that, unfortunately, this submission cannot be published in our journal. The reason is that we...
17 September 2023 7,410 0 View
I am standardizing a UV-VIS screening method that allows me to quantify provitamin A carotenoids. The results will be compared with samples quantified by HPLC, which is my Gold Standard....
06 September 2023 1,850 2 View
Dear Colleagues, It was not the first time that I paid attention to the fact that when uploading a new publication, the WG recognizes only a part of bibilographic references. Despite the fact...
02 August 2023 3,193 3 View
I would like to investigate the colourimetric properties of human eyes. The OneLight Spectra is ideal for this. Unfortunately, it has not been sold in Germany for over 10 years and the OneLight...
20 July 2023 8,045 4 View
Can anyone provide me with recent bibliographic references or studies investigating the structural, optical, and electronic properties of the perovskite material K2AgSbI6, particularly focusing on...
30 June 2023 5,949 0 View
