Omkar Panchal

4 Questions 23 Answers 0 Followers

Questions related from Omkar Panchal

What could be the reason I do not see knockdown at Protein level?

I am struggling with western blot since long time. Protein of interest is SATB1. I see very good knockdown on mRNA levels at 96 h. After knockdown, some cell lines start dying at 72 h while in...

03 March 2018 1,009 8 View

In a Denaturing PAGE (SDS), if the protein sample is degraded then do we see a smear or several bands near the protein of interest on western blot?

I forgot to add protease inhibitor cocktail before I lysed my tumor tissue samples for protein extraction. For 30 µg of protein I needed an exposure time of 2 seconds for the Actin protein as the...

01 January 2015 10,188 11 View

What should be the buffer composition and other parameters to be considered for Annexin V staining to work?

I tried Annexi V staining coupled with FITC flouroscent stain and analysed it by flow cytometry. I tried these two buffer compositions: Buffer1: 10 mM HEPES/NaOH, (pH 7.4), 150 mM NaCl, 5 mM KCl,...

11 November 2014 7,993 7 View

What is reference filter in a colorimetric assay? Why do we need that? How a reference filter wavelength is determined?

11 November 2014 9,386 0 View