I want to confirm protein localisation in Arabidopsis using fluorescent protein-tag. What are proper markers for plasma membrane, apoplast and cell wall?
Would anybody share such lines or plasmids with such markers?
Thank you.
Hi, I found this GFP-PIP2 marker that beautifully labels the plasma member in Arabidopsis. Here is a link to the reference Article Cell Geometry Guides the Dynamic Targeting of Apoplastic GPI...
I agree with the GFP-PIP2 suggestion above. Cell walls can be stained with various dyes (here is one option)
A protocol for combining fluorescent proteins with histological stains for diverse cell wall components - Ursache - 2018 - The Plant Journal - Wiley Online Library
Apoplast vs cell wall is going be a challenge. You could use immunochemistry (eg. Western blots) of culture medium to detect a secreted protein. GFP tagging may or may not work for direct visualization of secreted proteins as the pH of the medium will impact fluorescence.
You could try wall cross-linking followed by isolation and blotting.
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