What is GC/MS? I need details on gas chromatography mass spectrometry and how we apply this on plants. What is the purpose? Please provide a sample research paper if possible.
It is a fairly standard method in which the components of a mixture are separated by gas chromatography, (or alternatively liquid chromatography), and then the mass spectra of the individual components - or of selected components are measured. Direct mass spectra of a mixture - in particular a complex mixture - would often be very complicated and difficult to interpret. Spectra of the individual components are obviously much simpler, and usually the compounds involved - and even their structures - are relatively easy to identify. The use of the method is very widespread - for instance also in the airports, one is often subjected to subjected to a test using this method, searching for traces of drugs.
The GC device is generally a reliable analytical instrument. The GC instrument is effective in separating compounds into their various components. However, the GC instrument can not be used for reliable identification of specific substances. The MS instrument provides specific results but produces uncertain qualitative results. When an analyst uses the GC instrument to separate compounds before analysis with an MS instrument, a complementary relationship exists. The technician has access to both the retention times and mass spectral data. Many scientists consider GC/MS analysis as a tool for conclusive proof of identity.
GC/MS analysis, where the effluent to the GC instrument is the feed to the MS instrument, is in wide use for confirmation testing of substances. Drug testing, manufacturing quality control, and environmental testing are some typical uses.
For metabolite profiling in plants please look to e.g. Nat Protoc. 2006;1(1):387-96. Gas chromatography mass spectrometry-based metabolite profiling in plants. Lisec J1, Schauer N, Kopka J, Willmitzer L, Fernie AR. Basically the MS is usually used as two things here: a) if you ionize the GC eluent compounds with Electron Impact (EI) Ionization, you get spontaneous fragmentation patterns for most organic compounds that can be used for identifying the compounds of interest via database searching, provided your mixture is not overly complex so your spectra are reasonably "pure". b) The MS is also a good semi-quantitative detector that allows you for a comparison of samples (i.e. profiling).
Regarding the actual technique - by modern standards it is rather ancient (i.e. from the 70s/80s), so do not expect a lot of current literature. Textbooks of Analytical Chemistry are a more likely source for information, e.g. Skoog/Leary in case that is still around, or Budzikiewicz.
You have got answers here, just let me add that looking at your profile, in your case maybe the best app is to determine the possible bio-active components in plants.
here you have an example: http://www.jsrr.in/Vol.2%20No.3/2%20Thanga%205-9.pdf
Here you have two more, They are many examples that you can find.
GC-MS ANALYSIS OF PHYTOCOMPONENTS IN THE METHANOLIC EXTRACT
OF JUSTICIA WYNAADENSIS (NEES) T. ANDERS http://www.ijpbs.net/vol-3/issue-3/pharma/62.pdf
GC-MS ANALYSIS OF ETHANOL EXTRACT OF ENTADA PURSAETHA DC SEED. http://biosciencediscovery.com/Vol3No1/kalpana%2030-33.pdf
As any chromatographic technique it requires a bit of tweaking in order to find the right conditions of flow rate/temperature for separating correctly the components.
MS as a detector breaks up the molecules in segments the molar mass of which are then measured, ending up in a 'spectrum' of fragment frequency vs. weight, characteristic of a given molecule. The software then searches the best match in a library - with some error margin.
May I cite my own work done during my PhD, which used GC/MS to analyse wood extractives ?
Article The influence of wood aqueous extractives on the hydration k...
GC/MS stands for Gas Chromatography Mass Spectrometer.Basically It is used for separation of volatile organic compounds.
To be able to use GCMS for plants....first the sample(plant extract) has to be treated - sample clean up. Then, it depends on the component(s) of interest in the plant e.g you will need to know if the component is heat liable or not that will form the background for your using GCMS.If the component can be destroyed by heat then,GCMS is not the choice you need. Secondly you have to ensure that it can volatilize ,immediately it is injected into the column.
The purpose for using GCMS if all conditions are meet is: the mass spectrometer contains a library of standard spectrum,which will be compare to each of the chromatogram from your sample...and from the library, you will have various similarity search results for each compound from your sample showing their percentage of similarity.
The MS gives you the fragmentation patterns of each compound.
the GC gives the chromatogram (does the separation), while the MS (fragments each peak) and gives your results in bars. like vertical lines.
Gas chromatography–mass spectrometry (GC-MS) is an analytical method that combines the features of gas-liquid chromatography and mass spectrometry to identify different substances within a test sample. Applications of GC-MS include drug detection, environmental analysis, explosives investigation and identification of unknown samples and phytochemicals. It has immense and wide application in plant sciences e.g. studies of fragrance and flavouring compounds, fatty oils, foods and beverages, etc. that contain numerous compounds, some naturally present in the raw materials and some forming during processing. GC-MS is extensively used for the analysis of these compounds which include esters, fatty acids, alcohols, aldehydes, terpenes etc. It is also used to detect and measure contaminants from spoilage or adulteration. You may refer following links and the attached article.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3250032/
http://www.plantmethods.com/content/5/1/4
Dear Sir,
GC-MS is a technique used to quantify the bioactive compounds present in any sample and it is the first step towards understanding the nature of active principles in this medicinal plant and this type of study will be helpful for further detailed study.
With regards,
Dr.Tresina
GC-MS is a tool which helps in quantification of phytoconstituents present in plant samples.
What is GC/MS?What is the purpose?
yes what yogesh sir said is right
Gas chromatography–mass spectrometry (GC-MS) is an analytical method that combines the features of gas-liquid chromatography and mass spectrometry to identify different substances within a test sample. Applications of GC-MS include drug detection, environmental analysis, explosives investigation and identification of unknown samples and phytochemicals. It has immense and wide application in plant sciences e.g. studies of fragrance and flavouring compounds, fatty oils, foods and beverages, etc. that contain numerous compounds, some naturally present in the raw materials and some forming during processing. GC-MS is extensively used for the analysis of these compounds which include esters, fatty acids, alcohols, aldehydes, terpenes etc.
For details on gas chromatography mass spectrometry and how we apply this on plants. What is the purpose? sample research paper if possible.
In google lots of research article are there for application part
Gas chromatography is a destructive technique, after analysis sample is destroyed then why to connect with mass, how it will detect sample in GCMS?
Gas chromatography is a destructive technique, after analysis sample is destroyed then why to connect with mass, how it will detect sample in GCMS?I beleive due to destructive technique, there is no semi preparative and preparative column i believe in GC?
https://www.researchgate.net/post/Gas_chromatography_is_a_destructive_technique_after_analysis_sample_is_destroyed_then_why_to_connect_with_mass_how_it_will_detect_sample_in_GCMS
The GC works on the principle that a mixture will separate into individual substances when heated. The heated gases are carried through a column with an inert gas (such as helium). As the separated substances emerge from the column opening, they flow into the MS. Mass spectrometry identifies compounds by the mass of the analyte molecule. GC/MS can be used in both full scan MS or select ion monitoring (SIM) mode to cover either a wide range of m/z ratios or to gather data for specific masses of interest, respectively.
GC/MS targets small and volatile molecules. GC/MS is the analysis method of choice for smaller and volatile molecules such as benzenes, alcohols and aromatics, and simple molecules such as steroids, fatty acids, and hormones. It can also be applied towards the study of liquid, gaseous and solid samples.
It is a chromatographic device that is used to identify and analyze volatile compounds or non-volatile compounds that can be converted to volatiles. In the field of plants, especially medicinal plants, it can be used to identify the type of essential oils present in plants or to identify agricultural pesticides in plants.
Excellent submissions made by everyone.
It is an excellent technique for both quantitative and qualitative analysis of the various organic components in a plant sample.
But to begin you need to extract the components of interest from the plant(s) using appropriate solvent, clean up then analyze in GC-MS.
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