What is best strain of Agrobacterium rhizogenes for root transformation in soybean?

More Sangeeta Parthasarathy's questions See All

Sometimes we need to ask a single open ended question. How do we check reliability of that question ?

One of my questionnaires has one item: one open-ended question. Is there any method to check the reliability of one question?

23 September 2020 9,020 8 View

Can a non-binary sensing matrix be implemented using a Digital Micromirror Device?

In compressive sensing of images Digital MicroMirror Device (DMD) is used to implement the sensing matrix. DMD works in two states, on and off. This can implement a binary sensing matrix. How is a...

05 November 2019 2,096 3 View

I want to do DNA sequencing of nodule's rhizobium anybody can tell me from where i can do either in payment or free ?

i have nodules from different treated plants of vigna and i want to do molecular characterization of nodule's rhizobium

23 September 2018 5,317 3 View

Is there a closed form solution to the problem: Min_A norm(Y-AX)?

I need to find the matrix A that would minimize the expression: Norm(Y-AX), where Y is a m x L matrix, A is a m x N matrix and X is an N x L matrix, satisfying m

08 March 2017 2,061 6 View

Is there a closed form solution for minimizing a concave function in a convex region?

I need to find a entropy minimizing probability distribution under a convex constraint.

16 January 2017 2,774 8 View

How to decide the value of k in the kth nearest neighbor clustering method?

What is density linkage clustering?

16 February 2016 3,155 5 View

How do you check the quality of the serum isolated from mouse blood?

I am working with B6 strain of mice and need to study the markers for bone formation in the serum. But before doing that I would like to find out the quality of the serum, is there some standard...

22 February 2015 8,384 2 View

Are there any special requirements for Transmission Electron Microscopy (TEM) of magnetic nanoparticles?

TEM operators insist on embedding them in a resin. Will electromagnetic parts of the instrument attract them? My samples are not in powder form. Just a droplet of suspended particles.

24 June 2014 3,552 7 View

Can anyone explain the independent component analysis (ICA) algorithm?

I want to preprocess EEG signal using ICA algorithm.

16 June 2014 8,528 4 View

Can anyone help me with this problem of generating stable cell lines after puromycin selection?

After standardizing the optimized puromycin concentration (concentration at which nearly all cells are dead between 5-7 day), 48 hrs post transfection antibiotic was added to the cells at three...

16 April 2014 9,554 7 View

Covid 19 Pandemic ' impact on digital transformation?

Covid 19 Pandemic and digital transformation

22 February 2021 3,351 5 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Is the logarithmic transformation a necessary step in time series analysis ?

Hello, I'm checking if the log-tranformation of time series data is a necessary step; We all know the aim of such transformation, but in every study (in time series analysis) we found the authors...

15 February 2021 5,655 14 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View

How to produce huge amount of protoplasts from Fusarium graminearum?

Hi, all I am using some protocol for protoplast production from Fusarium graminearum (Fg), but I need them in a huge amount. Sometimes the protocol worked well but most of the time failed....

14 February 2021 2,286 4 View

How to measure 50uL of cells?

Hello, I am planning to do a transformation, and on the protocol it says "Scrape a 50 ml volume of yeast from the YPAD plate using a sterile inoculating loop or sterile toothpick". But I can...

14 February 2021 6,247 3 View

Mixed ANOVA with some normal and some non-normal data?

I am looking to run a 2x5 Mixed ANOVA to examine Sensory Processing Sensitivity in Highly Sensitive People (HSP) and Non_HSP. My Between-Subjects factor is HSP and Non-HSP. All participants tested...

11 February 2021 1,445 6 View

How to extract NLR genes from a database?

Greetings to all, I am not a bioinformatics person, but looking for a method that help me extract all NLR genes from benthamiana, tomato and soybean databases.. any suggestions so I can use my...

08 February 2021 4,557 2 View

What could be the cause to have an E.coli DH10B transformed with pUC18 to grow in MKL + ampicillin but is not LAC+?

Hello everyone, I did a transformation of E. coli DH10B using pUC18 plasmid. The bacterias did grow on MKL medium with ampicillin but the colonies showed a white/pink colour and the medium was...

08 February 2021 9,720 3 View

After transformation of Dh5alpha competent cells I found a cloudy white ppt before plating. Can anyone explain this?

After transformation of Dh5alpha cells by heat shock I grew them at 37 in 200microlitre Terrific Broth (because I had it at hand) and then before plating I found cloudy white ppt in the cell...

03 February 2021 7,867 3 View

Madhukar Baburao Deshmukh

Rhizogenes infects the plants, a portion of the Ri plasmid, the T-DNA, is transferred to the plant cell, integrated into the plant genome, and expressed as polyadenylated mRNA

Arbind K. Choudhary

Dear @Sangeeta Parthasarathy You can access the links given below; hope, these will be useful to you.

https://plantmethods.biomedcentral.com/articles/10.1186/s13007-021-00778-7

https://www.sciencedirect.com/science/article/pii/S2214514117300880

https://www.researchgate.net/publication/320339502_Soybean_hairy_roots_produced_in_vitro_by_Agrobacterium_rhizogenes_-mediated_transformation

https://www.scielo.br/j/pab/a/g3J7Cj4QMfyHrfJfXtnrPQQ/?lang=en