Details and uses of rpoB gene
It is a house keeping gene codes for recombinase protein unit-B. This is gene is used to draw phylogenetic relationship of bacteria as these are highly conserved in a species.
I am working on tobacco intervention and developing communication strategy, for that a questionnaire was developed, population is Indian tobacco users.
13 April 2024 9,324 2 View
I am using 100 and 120 nm latex particles and monoclonal and poly-clonal anti-crp antibody. i tried with Tris, Glycien, PBS, and MES buffer with different molarity (10 to 100 mmol) but not...
09 January 2023 1,467 3 View
We accidentally took the wrong filters into bsl3. We cant get anything out without being autoclaved. So do they survive autoclavable temperatures?
22 September 2021 9,026 1 View
We have this kit in our lab and I got a really good quality RNA last time when I used it on cells. I want to extract RNA from the virus supernatant, I was wondering if I can use the same kit for it.
15 September 2021 5,407 3 View
hi, I am trying to test the knowledge of teachers about the learning outcomes in science. Which aspects of learning outcomes can be included in my study?
22 February 2021 7,925 3 View
I have ethyle acetate extracts of photorhbdus bacteria which need to be identified but I am confused, when we are running TLC or HPLC to detect secondary metabolites, then d'nt know what to use...
19 January 2021 7,533 3 View
Hi, We are using Sievers M9 TOC analyzer for TOC measurements. We injected freshly collected water from Milli-Q purification system and observed TOC value is 105 ppb. Whereas the TOC value is 1...
21 April 2020 5,692 0 View
I am trying to optimize a TS for H2 + CN -----> HCN/HNC + H reaction using MRCI in MOLPRO. But I am getting the following error: In QSD: Caution, approaching trap region, itrap = In QSD:...
10 March 2020 2,693 3 View
As is known, for a pixel's value to be interpolated using bicubic interpolation the neighboring 16 pixel values are needed, but if a pixel lies at a corner of an image, and it's value needs to...
15 September 2018 5,457 3 View
How the statistical features can be extracted
07 April 2018 2,861 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View