I'm planning to collect Xylem Sap from Populus euphratica stem (5-7mm diameter), pressure use by XYL’EM embolism Meter, for analyzing Na+ and Cl− ions. Is there any accurate & simple method for sap collection?
Thank you for your suggestions.
Hello!
I did the same question and dr. Jorge gave me good ideas:
https://www.researchgate.net/post/What_is_the_best_method_for_determining_Sap_Ionic_Concentration_in_the_branches
Thanks dear Vitor barão!
The answer given by Dr. Jorge is very helpful.
I would check if John Sperry lab archive at Utah university has got quicker methods by using small stem segments put in a centrifuge tube at moderate speed. In this way you may collect several samples at once with a quite simple bench centrifuge. http://jxb.oxfordjournals.org/content/48/3/665
Hi,
yes the vaccuum extraction method work fine for species with medium size vessels (not for conifers, not for ring porous).
A good and easy alternative is the 'sap displacement' method: perfuse your segment with a colored solution (like Phloxine or eosine, but NOT Safranine !!!) under low pressure (10 kPa) and collect the sap flowing out of the distal segment. The colored solution will displace the xylem sap. Stop collecting when you see the colored solution flowing out.
Hope you enjoy your Xyl'em apparatus ;-)
Hervé
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