Alzheimer’s disease is the most common form of dementia, characterized by two pathological hallmarks: amyloid-β plaques and neurofibrillary tangles. It needs human neural stem-cell-derived three-dimensional (3D) culture system. More importantly, the 3D-differentiated neuronal cells expressing FAD mutations exhibited high levels of detergent-resistant, silver-positive aggregates of phosphorylated tau in the soma and neurites, as well as filamentous tau, as detected by immunoelectron microscopy. Inhibition of amyloid-β generation with β- or γ-secretase inhibitors not only decreased amyloid-β pathology, but also attenuated tauopathy.amyloid-β and tau pathology in a single 3D human neural cell culture system can recapitulate Alzheimer’s disease pathology in a 3D neural cell culture model should also serve to facilitate the development of more precise human neural cell models of other neurodegenerative disorders.

For screening purposes and for looking at the processing of APP use 7PA2 or 7WD10 cell lines.

For plant extracts and for cost effectiveness, preliminary ivitro studies using acetylcholinesterase or other enzymes responsible for Alzheimers would suffice but if you have isolated the compounds from those plants, then it would be good to carry out in vivo rodent model.