We need to run FTIR spec on our conjugates but cannot do so until we can properly dry them so that the solvent (PBS) does not interfere with the data collection. Does someone have access to this information readily?
Probably, for FTIR, the water molecules are obscuring the reading. Freeze (any other evaporative) drying will not remove the non-volatile salts in the buffer matrix.
For an antibody conjugate, a conservative drying method will work just fine, something like 200mTorr pressure with shelf temperature at -35°C should keep things happy. Method will take a while, depending on the formulation and fill volume, and the capability of the equipment in your lab.
A good general guide to freeze drying can be found here:
https://www.scientificproducts.com/resources/articles-tech-notes/basic-principles-of-freeze-drying
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