What histologic staining technique should be used to differentiate newly formed collagen from the existing collagen ?

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Steingrimur Stefansson

Hi Davoud, I am old school.

When I wanted to determine old collagen from cells depositing de novo collagen, I incubated the cells with 14C or 3H Ilu, Val, Leu.

Kinda primitive but it worked.

Davoud Kazemi

Dear Steingrimur

Thank you for your kind reply but I am more interested in histopathologic staining and evaluations.