In meta-analysis of binary outcomes, what should we do when the occurrence of outcome is zero in both groups?

In conducting meta-analysis of binary outcomes (in RCTs), in case the risk ratio has not been reported, what should we do with the studies that the number of outcomes occurrence is zero in both...

09 January 2021 2,802 5 View

How to calculate ratio of means and ratio of geometric means?

In conducting a meta-analysis, sometimes, some studies have reported geometric mean, while others have reported arithmetic mean. One solution for pooling these studies is calculating the ratio of...

11 December 2020 4,663 3 View

Is (Are) there any valid corpus containing geographical events queries?

Hi all, I'm currently looking for a question corpus to have a plausible number of spatial events queries. I found MS MARCO dataset in which I could extract a reasonable number of geographical...

08 December 2020 7,844 2 View

Do I have to assume sphericity or not when conducting two way repeated measures ANOVA in graphpad prism 8?

I am analyzing data obtained from a crossover study conducted on same animals evaluating the effect of two different anesthetic drugs on heart rate, respiratory rate, pulse oximetry, rectal...

28 September 2020 6,408 4 View

How to interpret arima model with regressors in r?

Hi there; I need to produce such an equation for a time series using arima model: y(t) = y(t-1) + a*x(t) + b*x(t-1) I know if I use for example arima (1,0,0) with x as the exogenous...

07 August 2020 7,438 6 View

Why some use seasonal ARIMA(p,d,q) (P,D, Q) instead of SARIMA?

Hello everyone. I am trying to use ARIMA model for a time series with seasonality. As I am new to this topic, I use the internet as my guide. My question is why anyone should use Seasonal ARIMA...

05 August 2020 219 4 View

How can I add stations to HBV-Light model?

Hi everyone. I've been using the original HBV model, so far. I might need to change it to HBV-light. Does anybody know how to add stations to HBV-light software? I can see there is this...

01 April 2020 1,032 7 View

How to define the parameters of Spatio-Temporal variogram models?

Hi everyone; I have a question regarding the parameters of variogram modelling. Does anyone know how to choose parameters such as Sill, Range and ... for the time, space and joint components of a...

10 February 2020 2,381 6 View

How to change tunit in VariogramST (R)?

Hi there, I am trying to use VariogramST function in R, for spatial-temporal kriging. The values I am working on, are monthly based; while in the function VariogramST, the predefined "tunit" is...

27 January 2020 5,986 3 View

The effect of number of antennas in multistatic architecture for imaging and SNR?

I believe by using greater number of antennas in simulation, SNR would also be improved. However I have no proof for that. Are there any equations that support my idea?

25 September 2019 4,890 3 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...

28 February 2021 552 3 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View