I noticed some problem with hplc. As you can see in the photographs, negative peaks and strange chromatograms appear. There is no change in pressure during the run. What do you think could happen? The system is Thermo Scientific Ultimate 3000
You may be getting air bubbles in the detector. Make sure your degasser is working properly and you have no leaks.
Another possibility is a failing deuterium lamp, but I would investigate the degasser and check for leaks first.
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