What gel composition can be used to analyse mAbs aggregate of 700 kDa using native gel electrophoresis?

11 September 2016 1 627 Report

I am using polyacrylamide gel electrophoresis. I want to analyse 700 kDa aggregates. I don't have gradient maker. I have tried different concentration of (stacking gel and resolving gel) as (3%,5%) and (3%,6%) but I have not observed any band. Can you help me with gel and concentration I can use ?

Thank You

Adam B Shapiro

You may want to try an agarose or agarose-acrylamide composite gel for such large protein complexes. Here are some references:

http://cshprotocols.cshlp.org/content/2011/7/pdb.prot4558.short

http://www.ncbi.nlm.nih.gov/pubmed/15979554

http://bio.lonza.com/uploads/tx_mwaxmarketingmaterial/Lonza_BenchGuides_SourceBook_Section_XIII_-_Protein_Separation_in_Agarose_Gels.pdf

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