What factors determine the complete conversion in the synthesis of nanoparticle?

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I noticed some white growth in the phosphate buffer that was prepared just a week ago. What could be the cause of this, and how can it be resolved?

The buffer wasn't filtered.. Could this be the cause? Can we autoclave phosphate buffer to resolve this issue?

10 June 2024 3,970 2 View

For co-culture HUVEC and muscle cell and use angiogenic and arteriogenic growth factor, what are the markers represent stable blood vessel formation?

We have seen in the literature that Ang-1 can be considered as one of the markers. We need information about few more markers.

01 June 2024 9,650 1 View

Notch 3 can be considered as marker for stable blood vessel formation?

01 June 2024 9,788 0 View

I need to know the markers for stable blood vessel formation?

Both angiogenesis and arteriogenesis are needed to make stable blood vessel formation. Ang-1 is one of the markers for stable blood vessel formation. Can you please suggest names of few more...

01 June 2024 1,912 1 View

How to solve Fuzzy Boundary Value Problem by using Fuzzy Laplace Transform Method?

There are so many papers available to solve initial value problem by using Fuzzy Laplace Transform method. But need solution for the boundary value problem by using the same method.

29 November 2023 9,727 2 View

Does stretching an ultrashort laser pulse by passing through a medium (like SF11) introduce new frequency components in it?

I am stretching ultrashort laser pulses by passing them through an SF11 plate. The pulses are stretched due to the introduction of different delays to the group velocities of the various frequency...

28 April 2022 5,144 5 View

ReLeSR or manual passaging at passage 0 after iPSC generation?

I have generated iPSC colonies from peripheral blood, today is Day14 post-transduction and the colonies seem to have acquired nice borders and distinct iPSC morphology (images attached). There is...

16 December 2021 7,082 1 View

Can we calculate MOI of lentivirus from copy number/ml quantified using qRT-PCR?

I have calculated the copies/ml of my lentiviral particles using qRT-PCR. Now, to calculate MOI, MOI = plaque forming units of virus for infection/number of cells. In this case, can I equate...

10 November 2021 4,165 4 View

Do these iPSC colonies look okay?

Hi, I am new to iPSCs. I have attached some pictures of newly reprogrammed iPSCs, now at passage 3. They look okay to me, with defined boundaries, but they are not perfectly round, instead have...

01 March 2020 418 18 View

Why are my transduced PBMCs not attaching to Matrigel?

I am folowing the Cytotune 2.0 protocol for feeder-free reprogramming of PBMCs. Everything goes well till 4-5 days after transduction by which time the transduced cells begin to form bigger...

04 February 2020 1,733 14 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,328 0 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,891 1 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,973 3 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,418 1 View

CAD File of human's & rat's respiratory airways ?

Dear all, I am working on particle deposition in human's & rat's respiratory airways using CFD and I am looking for the 3D CAD file for my simulations (STEP or IGES format). If somone has such...

29 July 2024 1,090 2 View

How can I use DFT-D3 correction ( Siesta version 5.0.1)?

Dear Siesta Users, I installed Siesta 5.0.1 and I want to use Grimme's D3 correction. According to the 5.0.1 manual, I could use a few parameters to include the D3 correction in the...

27 July 2024 5,747 0 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,737 0 View

Mechanochemistry: ball to powder mass or ball to powder volume?

I run mechanochemical reactions that involves the use of a large amount of catalyst (1:1 molar ratio to the reactant). When I try to understand the effect of amount of catalyst on the reaction, I...

23 July 2024 9,937 1 View

How to eliminate the carry-over effect in GC-MS/MS system?

Hi, I am a PhD student working at Jagiellonian University, Faculty of Chemistry. My work is related to the development of new analytical methods for the quantification of short-chain fatty acids...

22 July 2024 5,491 6 View

I want to buy Hydrothermal Synthesis Autoclave from any European company. Can anyone suggest any company inside Europe?

Thanks

22 July 2024 1,142 3 View