How to use protein A G beads to purify antibody?

Secretory universal influenza antibody CR9114 were successfully expressed by adenovirus vector ,then we used protein A G beads purify CR9114.But the molecular weight of the purified antibody under...

22 February 2021 4,318 2 View

Amorphous silica XRD peak at around 10 degree?

21 February 2021 3,417 5 View

How could I get the P-value from the mean, SEM, and n of different groups in the t-test?

How could I get the P-value from the mean, SEM, and n of different groups in the t-test without the original data? For detail, I would like to know the P-value between BMP-2: 50ng/ml group and the...

06 February 2021 6,539 4 View

What mechanism is involved when chitosan is coated on magnetic nanoparticles?

Hello! I am currently working on coating of chitosan on magnetic Fe3O4 nanoparticles. I am wondering how this coating process happened? i.e. through what mechanism that chitosan can be coated on...

13 January 2021 5,657 5 View

The impact of different poisson's ratios when analysising field vibration?

The Poisson's ratio of soil is quite difficult for us geo-engineers to obtain. I came across a problem when I tried to determine the parameters in a FEM model. For the saturated clay in...

02 January 2021 8,971 3 View

Is it possible to deposition a large area dielectric layer(mm scale) on the wet transferred graphene?

I want to deposition a dielectric layer on top of the CVD wet transferred graphene. The purpose of the dielectric layer is to uniformly cover the graphene and enable the top-gate modification of...

14 December 2020 7,693 2 View

Is the fluorescent protein must be totally photobleached in FRAP assay?

Hi I have tried to measure the mobility of a protein of interest by the FRAP assay. The protein was fused with GFP. I overexpressed the fusion protein in Hela cells. However, when the area of the...

14 December 2020 6,567 1 View

Is there some effective transfection reagents or methods for transfecting plasmids to ExpiCHO-S cells?

Dear all, We want to transfect pCDH plasmid or other plasmids to suspension ExpiCHO-S cells and express our target proteins, and we used the transfection reagents PEI. Unfortunately, there...

26 November 2020 8,323 3 View

No title

The cell I used is 293T.I want to it can grow on the biocompatible materials,like agarose or alginate gels.But the shape of the cells is not good.In the T25 flask you will find the cell like...

26 November 2020 1,009 2 View

Again, what are the bands of extracted plasmid?

I used Trans1-T1 phage resistant chemically competent cells to extracted lentiviral gRNA expression vectors. And there were bands near 2000bp and smear on 1% argrose gel. Did the smear come from...

25 November 2020 858 2 View

Could the "incoming radiation (net radiation)" values be negative?

Could the "incoming solar radiation" values be negative? What is the difference between "global solar radiation" and "incoming solar radiation"? Which one might be measured negative values?

21 February 2021 7,726 9 View

Is the box plot of climatic indices better illustrate from daily data or annual data?

Hello, I hope you have a good time. I work on a research project about temperature indices. Due to the high number of indices, I only work on tables and maps on an annual time scale. In other...

19 February 2021 9,742 2 View

How to do marking of reference year in urbanization up sprawl mapping ?

I am performing mapping of urban up sprawl for 5 cities in Pakistan using advanced geospatial methods with satellite imagery for 25 years (1995-2020) with a 5 year interval. Can someone enlighten...

19 October 2020 6,097 3 View

Which software is best to plot paleoclimate data with high resolution?

Dear All, I'd like to plot high-resolution figures of paleoclimate data, Please suggest the best and most friendly software. Advance thanks, Saravanan

25 July 2020 3,003 6 View

What programming language can we recommend to a paleoclimatology student?

I will use climatological models in my research work but I have not yet mastered any programming language, now I would like to have advice on the choices to start.

21 June 2020 9,595 9 View

What are some of the free sources where i can download paleo-climate data ? What things you take into considerations while processing paleo data ?

Greetings, I am planning to work with open source paleo-climate data for a thesis, so far the only source for these kind of data i know is : https://www.ncdc.noaa.gov/paleo-search/ Is there...

28 May 2020 9,084 14 View

How may we make the regions of the country Mathematically with respect to climatology?

DIfferent Mathematical Techniques are being used for regionalization. For example in different references, the authors regionalize the area across the country under different climates.

10 April 2020 1,829 17 View

How to convert data with a Lambert Conformal Conic projection?

I downloaded Cordex historical data from Copernicus CDS (https://cds.climate.copernicus.eu/cdsapp#!/dataset/projections-cordex-single-levels?tab=form). Data are in NetCDF format. When I read the...

01 February 2020 840 3 View

Do anyone know have scripts ot packages to detect atmospheric front automatically based on reanalyisis or climate model data?

Our group is trying to investigate the relationship between regional precipitation (especially over regions of east Asia monsoon and South Asia Monsoon) climatology and atmospheric front? We aim...

08 January 2020 9,945 3 View

How to create monthly gridded rainfall data set based on point-based observational data?

Hello all, I want to know how to create MONTHLY gridded data set based on point-based observational data (synoptic stations) for the rainfall and temperature. I was read some article about...

11 December 2019 4,414 10 View