I am currently planning a research based on the evaluation of the potential use in the feeding of ruminants of the by-products of the milling industry (e.g., dry pasta, biscuits and others). In this regard, would I need to know which in vitro tests other than gas production can be performed to determine the ruminal degradation kinetics of starch. More generally, which in vitro tests can be used to define the digestive utilization of starch? Furthermore, the excessive starch content of the samples can influence the outcome of other in vitro tests, such as the three-step procedure of Calsamiglia and Stern? I thank everyone in advance.
I think you also need to screen the diversity and change in ruminal microflora especially protozoa and fibrolytic degrading bacteria in rumen microflora
If you are interested in the ME or NEL of such products, you can use the Hohenheim Gastest. The gas measured, together with some crude nutrient analysis correlates very well with the energy content. No need to look into gas composition or alike. This is sufficient for diet formulation as long as you don't overdo it with the inclusion rate.
I think that the best answer is to look at the research of Mary Beth Hall
Dear colleague
It could be nice to know best the planned objectives of your research. Anyway, I have not any issue with in vitro gas production test, you have to check if in practical condition some starch could scape from rumen as I imagine that many of those by product are very water soluble. Another approach can be mix with different proportions of an standard foraje. But as I wrote, a better definition of research objectives will be more helpful to see what to do. Best regards
Redimio
Francesco Serrapica Making digestibility in vitro for amylaceous materials intended for ruminants is one of the most inaccurate activities that exist in nutrition of these herbivores. For what reason? The microflora of the rumen greatly varies its ability to degrade carbohydrates with the diet received and the amounts or proportions thereof. The more starches in the diet, the more degradation thereof and the lower pH, with this the less amount of cell wall degraders, and the higher the fiber, the greater the amount of cellulolytics, the higher the pH and the less degradation of amylaceous compounds. Therefore, it is better to make measurements in situ, with nylon bags, feeding the animal with the expected base diet to predict the use of these carbohydrates of high degradability.
Colleagues:
I still thinking that gas in vitro technique can be aslo a choice, I do repeat: a better definition of research objectives will be more helpful to see what to do.
Regards,
Redimio
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