I am facing the same problem with my LAMP assay. I have optimized everything, and I was doing LAMP PCR, but suddenly, I am seeing positive results in NTC. The pattern of NTC and reaction is exactly similar. Also, the trend is not continuous, sometimes my NTC comes clear, and sometimes it shows bands. I have changed my reagents and fumigated the room, using filter tips, but still facing the on-and-off NTC signal. I am analyzing the results by gel electrophoresis. The pre and post-PCR amplification areas are also different. Reaction conditions are 61 degrees for 60 minutes. I have tried reducing the incubation time but the results are not replicable. if anyone can help, please contact me at my email id: [email protected]

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