In 14 days old culture of Chlorococcum (Green algae) I have found Phormidium within the culture. Please give me some suggestions as to how I could remove the cyanobacteria from the culture.
Dear Slobodanka, I already tried the solid plate technique. When the unialgal colony were picked from the plate and put into the liquid medium for culture, it was contaminated again with phormidium. I think there are still some spores or hormogones of Phormidium which were germinated after adding them in liquid suspension.
Use a broad spectrum antibiotic..phormidium being a cynbact will get eliminated from the solid agar plate
Treatment with hydrogenperoxide might do as well. Cyano's are very susceptible to tiny amounts of H2O2, compared to eukaryots.
Best wishes, René
hope this could help.
Recently was trying to download a paper mentioned below but failed to do so. so couldnot send the attachment. In this paper there is a table which shows shift from BGA to Green algae.
Importantly addition of CO2 and available nitrogen, pH maintained at 5.5-7.5 with HCL do show shift of BGA to Green algae in culture. if you have time, then can try this out to check whether BGA growth can be controlled.
Shapiro J. 1990. Current beliefs regarding dominance by
blue-greens: The case for the importance of CO2 and pH.
Verh. Internat. Verein. Limnol.
24: 38–54.
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