Agarose EMSA for aptamer binding: no bound complex band?

Hey there, Due to limited resource, we are trying to assess the interaction of a 50mer aptamer with a 25kDa protein domain. The protocol we are using is unusual in that the gel is 2.5% agarose...

23 July 2020 8,192 8 View

Design of PCR primers for extremely short oligonucleotides?

Hello there, hoping you are well, I have reached a point in my current project where I need to amplify very very short (50mer) non-coding ssDNAs. I understand the options I have available...

20 July 2020 1,558 5 View

Can the overall similarity of a standard mass spectrum to that from an unknown be used for identification without using the fragmentation ion ratio?

Here is situation: Compound is run on GCMS give a mass spectrum. Old standards are used from a library. The identification is made by observing with eye that the two OVERALL patterns are...

11 February 2020 3,965 3 View

What operations are necessary for a proper identification of a compound using optical microscopic methods?

It is my understanding that the forensic identification of a controlled substance using optical microscopy requires more information that just the classification of the type of crystal formed....

27 January 2020 5,751 2 View

What software is available to model the impact of design change in complex products?

This question relates to research into the management of engineering design information and the impact of innovation. Complex products such as aircraft, trains and ships are high-value, long-life...

18 December 2019 316 3 View

What software is available to model the impact of design change in complex products?

Complex products such as aircraft, trains and ships are high-value, long-life assets that are typically managed as a fleet over a period of 20 to 30 years. Managing technology upgrades...

18 December 2019 4,710 3 View

cannot delete question?

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27 April 2018 5,230 4 View

In SPSS, how can I adjust data to remove the effect of a covariate?

In brief overview, I have grown some plants and measured plant height (amongst many other traits), but my plants have been damaged by frost. I gave each plant a frost score between 0-5 as a...

05 March 2018 5,520 4 View

Haemoxygenase-1: does anyone have expertise for what solvent to use to store this protein in at -80 degrees?

Hi We recently started doing some ELISAs to look for levels of this protein, but are finding that reconstitution in water (as instructed by manufacturer) probably didn't completely solubilise the...

09 May 2017 7,815 1 View

What tools are there to detemine the genomewide binding profiles C2H2 Zinc finger proteins with allelic PWM?

27 December 2014 7,108 3 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Have you ever experiencing air vent trouble in fermentation process?

Dear all, My research group is currently working with a 12-litre fermenter. Somehow, if we do the fermentation for more than 20 hours, the process will be more prone to contamination. Our current...

23 February 2021 1,908 2 View

Is there any available free access High Performance Computing (HPC) servers with GROMACS?

I would like to run a Molecular Dynamics (MD) simulation using GROMACS in a high-performance computing server. Is there any freely available one out there? If yes, please do not fail to make...

16 February 2021 2,593 3 View

Can I use the CHARMM GUI input files for Gromacs in HPC?

Good day everyone! I am a Biochemistry undergraduate in the Philippines and to be very honest, we have no background in bioinformatics since we really focused on wet lab techniques + theory. Due...

15 February 2021 3,422 4 View

What will happen when we assume an upper bound of OFV lower than the global minimum OFV ?

if we assume 20 is the upper bound of an objective function (OF) based on permutation a square matrix (nxn) , The minimum value of the OF=22, then we run a random operator to compute the OF by...

14 February 2021 6,056 5 View

I'm looking for HVAC relevant topic for thesis?

Heating Ventilation Air-conditioning System related thesis topic for thesis

09 February 2021 4,215 5 View

Infinite order of Laguerre?

If Lmn(x) is the Laguerre polynomial of order m and degree n Is there a means of computing Lmn(x) for 1) m goes to infinity 2) n goes to infinity

08 February 2021 6,320 4 View

What is the nature of this algorithm?

What is the kind of this algorithm; Input : square matrix Objective function = minimization of total movements cost of matrix based on permutative manner Constrains : Lower and upper bound of the...

04 February 2021 2,050 3 View

Do have new reseach that you wish to publish a chapter on for free: Areas now Blockchain and cooperative computing?

03 February 2021 4,497 6 View