What causes mixture separation into two phases when adding p-Anisidine reagent (p-anisidine in glacial acetic acid) to fat solution (oil+iso-octane)?

08 August 2018 0 10K Report

I am conducting the p-Anisidine value determination on sunflower oil samples. However upon the addition of p-Anisidine reagent (p-Anisidine dissolved in glacial acetic acid) to fat solution ( oil + ios-octane), the mixture shows separation into two phases (even after shaking). What could have caused this knowing that there was no water at all during the whole experiment?

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