Fish??

That means cured

Kris Valerie Silmaro Navidad

Simply filter the supernatant/water using a mesh with a pore size suitable for microplanktons, preferably 5-20 microns. Then gently rinse/wash the residue onto a watch glass or petridish for your perusal. The following link should help you a lot:

Regards,

Dr. Abhishek Mukherjee

Hi Valerie. In our lab work in envi.tox last year under dr joey martinez, we lassoed nematodes with forearm hair tips glued to a short piece of bbq stick.

First remove the excess water with the help of a fine-tipped dropper or simply a disposable syringe with the smallest possible needle. I usually use insulin syringes. Then, with the help of a nematode fishing (also called picking) needle you can bring the nematode near the surface of water and then give a quick jerk just to break the surface tension of the water. While doing the above-mentioned process you should keep focusing on the nematode.

The picking needle can be prepared by using an empty ballpoint pen refill. Take a piece (nearly 1 inch) of hair (horse tail) and fix on the pointed tip of the refill with the help of glue or nail polish. Now cut the extreme tip of the hair obliquely so that you get a finely pointed tip.

To transfer individual worms to a slide, try an Irwin loop. An Irwin loop is basically a small loop made of twisted wire and mounted on the end of a small dowel. Google "Irwin loop" to gather more information.

Thanks so much to everyone who gave out suggestions :) due to limited availability of materials in our lab, we decided to use forearm hairs attached to the tips of dissecting needles (attached using nail polish) as suggested by Sir Danilo A. Gualberto to isolate nematodes from remaining sediments (after sieving). we then used a micropipette set to 8 microliters to collect the nematode and place it on a glass slide for viewing. Hope this helps someone out as well :)