What can be used to fix the mobility of E. coli to acquire the images by using fluorescence microscope
Do you mean immobilization of E. Coli cells for fluorescence microscopy? Depending on the surface you try to attach cells, there are basically two mild options. One electrically, if you know their surface charge, the opposite charge coating might help (e. g. PLL or PGA). The other is to chemically bind some specific surface molecules. Hope this helped.
Dear Attila,
Thank you so much for your response.
Yes, I wanted to know about any adhesive kind of material which can immobilize the E. coli without any interference to the cells, so that I can take the fluorescence microscopic images.
Because, once I tried but it could not possible due to the rapid movement of E coli in aqueous medium.
Respected Sir (Liam Good),
Thank you so much for your kind concern.
With best regards
Dr Arun
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