15 February 2022 3 5K Report

I use some applications, but I seek more useful options. I wonder you're the applications that you are used to and your experiences with them. Thanks for your replies in advance.

More İlker Dere's questions See All
How to concentrate DNA in extract?

Dear Researchers I used a Kit for nucleic acid extraction ( magnetic bead) to extract DNA in cell pellet and plasma. The concentration of DNA are low in most cases and I need to get a protocol or...

14 January 2024 4,266 2 View

How can I more effective use Google Academic Alerts to follow my research field?

Dear colleagues, I have used Google Alerts for 4 years and it has worked only 4 or 5 keywords. I add some more keywords, I only receive some news about the topic per week. Where do I mistake?...

13 February 2022 7,803 3 View

Is it possible to differentiate the oxidation states of chromium element and determine the hexavalent chromate content of a material precisely?

I am working on a project regarding the removal of hexavalent chromate and I first need to differentiate the oxidation states of chromium. Is there any instrumental methods to be able to do this?

06 May 2021 1,475 4 View

What kind of a relation is there between the cement hydration and the concrete properties?

How are the concrete properties changed by the differences in hydration?

24 May 2020 4,130 10 View

Is there an existing DICOM to MCNP input deck converter?

I am wondering if there is a DICOM to MCNP converter to import CT images into MCNP input decks? I am aware of Scan2MCNP but it seems to have been discontinued for some reason. If there is no such...

10 October 2018 2,911 3 View

How can I extract thiodiglycol from human urine by using C18 SPE?

I am trying to find thiodyglycol from the urines of the mustard attack victims of ISIS. I am using the C18 solid phase extraction column, but I used methanol to wash the elute and was...

04 December 2016 1,916 0 View

How much of an anticipatory postural adjustment (APA) is obstacle crossing during walking?

I have knowledge, not so deep, about APA, when there are internally or externally originated perturbations during standing still or during gait initiation. But when I think about obstacle crossing...

30 June 2016 6,147 4 View

Simulation for ANN with matlab?

Hi,  I am forest biometrician in forest faculty,  I have studied for predicting various tree and forest attributes by using ANNs, I can apply different ANN training networks such as feed-forward...

31 May 2016 4,898 2 View

What is the method to obtain homogeneous dispersion of enzyme particles in chitosan solution?

We observe agglomeration of tyrosinaze enzyme (solved in PBS) after interaction with chitosan solution (prepared in 0.1 M HCl). Is there any suggestion to prepare homogeneous chitosan enzyme mixture. 

06 December 2015 1,136 3 View

Does an additional kozak sequence located upstream of a cloned ORF containing its own kozak sequence influence the expression of the protein?

For our agrobacterium mediated plant transformation study, we cloned our gene of interest into a binary T DNA vector called pGFPGUSPlus. As the vector construction strategy, in the pGFPGUSPlus...

21 October 2015 8,437 2 View

Similar questions and discussions
The Optimal Experimental Approach for Gene Function Analysis?

What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...

04 August 2024 6,265 1 View

How to we see transcription activity of the bhlh79 family is at the N-terminal or C-terminal?

I'm a PhD Student. From Northwest Agriculture and Forestry University China. My Problem is this I start my work on bhlh79 transcription factor gene. I do my Y2H 5 time but the colonies appear on...

30 July 2024 1,412 8 View

How do you assign allele numbers and sequence type after amplifying housekeeping genes by PCR for MLST?

How to assign allele numbers and sequence type after amplifying housekeeping genes by PCR for genotyping of S. pneumoniae strains by MLST using bioedit ?

19 July 2024 5,239 2 View

What happens in snapTotal- seq?

What happens in snapTotal- seq? (Single-cell Total-RNA Profiling Unveils Regulatory Hubs of Transcription Factors https://www.nature.com/articles/s41467-024-50291-3) In this protocol, several RT...

18 July 2024 10,010 0 View

Is it possible to generate a (semi-)permanent "transcription bubble" within a replicable plasmid?

I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...

07 July 2024 8,275 5 View

Is DNase I (RNase-Free) necessary to be used before cDNA synthesis?

I want to isolate RNA from bacillus subtilis using the QuickRNA Fungal Bacterial Miniprep Kit from Zymo Research and further reverse transcriptase to cDNA using the ReverTra Ace qPCR RT Master Mix...

27 June 2024 2,951 5 View

Can you publish your dissertation after the data has been destroyed?

Hello, Can I publish my dissertation even though the original data has been destroyed in line with my university's ethical procedure? Data was to be destroyed upon completion of my degree. I'd...

25 June 2024 9,580 0 View

How good can CI users localize sound?

It is probably pretty variable between CI users, any ideas?

24 June 2024 6,879 1 View

I'm stimulating the shaffer collateral but do not want to generate LTP, what herz am I limited to?

I'm a graduate student at UWM focusing on electrophysiology. I'm trying to perform Shaffer Collateral stimulation of CA1 pyramidal neurons. If I were to have a protocol with 6s of no stim, 2...

18 June 2024 319 0 View

How many interview participants should be quoted/mentioned in analysis in a mixed-method design?

Hi Everyone, I'm conducting research on culture shock using a mixed method design (Questionnaire + Interview). I have 60 Participants in Questionnaire and 10 participants (out of 60) in the...

13 June 2024 5,190 1 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close