Which is better for minimizing cross-contamination and why? Is an o-ring essential for you and why?
Hi Friends, I have been having a nightmare genotyping these transgenic mice. What I have are heterozygous mice with a few knock-in point mutations. Originally, we used the same WT reverse primer...
10 November 2014 6,476 10 View
Hi friends, I have been working with a strain of transgenic mice with a mutated gene. The mutated region was used as the mutant forward primer. Therefore, anytime I get a PCR product, I am...
07 August 2014 4,533 8 View
I would like to test for ubiquitination in my samples, but don't want to end up with a smeared blot. Can anyone recommend a good antibody to use? Or does any company make anti-ubiquitin MKP-1...
04 May 2014 1,560 1 View
PVDF membrane - only stripped once.
31 December 2013 4,127 5 View
I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...
10 August 2024 3,629 1 View
We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.
08 August 2024 3,161 0 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Samuel Morse, the inventor of the Morse Code, understood that certain letters in the English language occurred more frequently than others (Gallistel and King 2010). To deal with this, Morse used...
01 August 2024 4,456 2 View
Hey All! I am wondering what might be wrong with my band structure. I did the calculations using VASP and plotted the results using Origin. Although I have tried changing various input...
25 July 2024 2,920 11 View
It is being seen that Editors of some reputed Journals are putting on hold the manuscripts and not updating review states even passing more than six months from submission. Researchers are...
24 July 2024 8,596 2 View
90% of museum objects worldwide are in storage.
21 July 2024 8,038 6 View
Our lab has some radioactive cell samples that need to be kept frozen for at least 30 days before flow analysis. For some reason, the samples stored in liquid nitrogen did not perform as well as...
15 July 2024 9,975 1 View
I performed CV of Cu-MOF in a buffer solution of pH 8, and we did not find any peak corresponding to Cu2+ to Cu+. What is the reason behind this? Please give your valuable suggestions.
08 July 2024 2,388 4 View
What could be the reason for mixotrophs to be yellow too instead of green? Thank you
08 July 2024 594 2 View